Sequences from confirmation amplicon


Confirmation sequences for line 727F10 and hit At3g12740 are shown.
Line ID 727F10
Locus At3g12740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4050810
Primers used for PCR 23wm - 8474, show primer details
   
Sequence Name 59-K118635-0022-727-F10C-23wm
GenBank Accession LN510111 [GenBank]
Sequencing primer gene specific primer, 23wm: 5'-TTCTTCCCACTGAAACTGTATGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K118635-0022-727-F10C-23wm
TCTCACCCTTCTCCAAGTCACTCTCTATCTTCCCGTATAATTTTCTAAATGTTGGCAACG
CAGCAGTCCTCATCCACACGATCAGATCCTCTTGATCACTCAACTGAAAGAGATAGAGAG
AAAGGGAGACTAAGTTAAGCGTTTTGCAAAGGATATGACTGGGTCTAAGACTGTACTGTG
GTATTAAACGCTTACTGGTTTATTTGGATCTAGACTGGCACCACCAGTGAGATTGCCCTT
CTGAAAGTTCTTGGGAAACACATTTTTCCCAAACTTGTGTTCCTTGTCGCTCTTCCATGC
AATTCCTTTCTTGTTTACCGTTAAACCCTGGTTATTTCTTGATAGAACGTATGTGTCATT
AAAGAGACTCCAAGCAATTAGACCACATGGCACAATTGGCTGCCCACCAAAATCATCCTC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGC
   
Sequence Name 19-K118634-0022-727-F10C-8409
GenBank Accession LN494971 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K118634-0022-727-F10C-8409
TTGAAATATCCTGAGGATGATTTTGGTGGGCAGCCAATTGTGCCATGTGGTCTAATTGCT
TGGAGTCTCTTTAATGACACATACGTTCTATCAAGAAATAACCAGGGTTTAACGGTAAAC
AAGAAAGGAATTGCATGGAAGAGCGACAAGGAACACAAGTTTGGGAAAAATGTGTTTCCC
AAGAACTTTCAGAAGGGCAATCTCACTGGTGGTGCCAGTCTAGATCCAAATAAACCAGTA
AGCGTTTAATACCACAGTACAGTCTTAGACCCAGTCATATCCTTTGCAAAACGCTTAACT
TAGTCTCCCTTTCTCTCTATCTCTTTCAGTTGAGTGATCAAGAGGATCTGATCGTGTGGA
TGAGGACTGCTGCGTTGCCAACATTTAGAAAATTATACGGGAAGATAGAGAGTGACTTGG
AGAAGGGTGAGAACATACAAGTGACTCTGCAGAACAACTACAACACATACAGTT

Last Updated on 06.12.2018 16:11