Sequences from confirmation amplicon


Confirmation sequences for line 727F11 and hit At1g19700 are shown.
Line ID 727F11
Locus At1g19700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6810441
Primers used for PCR 08cz - 8474, show primer details
   
Sequence Name 18-K109029-0022-727-F11C-08cz
GenBank Accession LN494340 [GenBank]
Sequencing primer gene specific primer, 08cz: 5'-CCTCTGTACCAAGAAGAACCAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K109029-0022-727-F11C-08cz
TAGTGATGGGATTGGAGAATCTGCTTTCTTGCTGGTTGCTTCCGCTAATGTAACGGTAAT
CATCAACACCAAACCCGTGATAGTTCATCAGAGTGTCGTAATCCGATGAGCGGTTTATCT
GTTGTTGCTGTGGGTCGTTATCATTGCCTAGAACACGATCTGGTTTGGTTTCTGATGAGA
AGACAATGTTTGTTGTGTCTGCAGATGAATAAGCGAGGTTGCTGTTGTTGTTGTTCTCTT
GCTGCTGCTGCTGCTGCGATGAGGAGTCTTCTTGGTTTGTGCTGTTGGGTTCTTGGGAAG
ATTTGGAGATTAGTAACTCAGATTCATCACCAAACTCTTCTTTATACATTTCTTCGATCA
TCGGTTTCCACAGACGAACTCTCGCGTTTATGAACCAATTAGCAACCTAATCATTTAGCA
GAGAAACCAGAATTATTCAAGCAGAACACAAAGGGTGCAAATTTTAAGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 02-K109028-0022-727-F11C-8409
GenBank Accession LN487068 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K109028-0022-727-F11C-8409
TCGGACTAAATTTGCCCCTTTGTGTTCTGCTTGAAAATTCTGGTTTCTCTGCTAAATGAC
CATGTTGCTAATTGGTTCATAAACGCGAGAGTTCGTCTGTGGAAACCGATGATCGAAGAA
ATGTATAAAGAAGAGTTTGGTGATGAATCTGAGTTACTAATCTCCAAATCTTCCCAAGAA
CCCAACAGCACAAACCAAGAAGACTCCTCATCGCAGCAGCAGCAGCAGCAAGAGAACAAC
AACAACAGCAACCTCGCTTATTCATCTGCAGACACAACAAACATTGTCTTCTCATCAGAA
ACCAAACCAGATCGTGTTCTAGGCAATGATAACGACCCACAGCAACAACAGATAAACCGC
TCATCGGATTACGACACTCTGATGAACTATCACGGGTTTGGTGTTGATGATTACCGTTAC
ATTAGCGGAAGCAACCAGCAAGAAAGCAGATTCTCCAATTCCCATCACTTACACGACTTT
GTTGTGTGATATTATCTGGTTCTTCTTGGTACAGA

Last Updated on 06.12.2018 16:11