Sequences from confirmation amplicon


Confirmation sequences for line 729B04 and hit At1g53320 are shown.
Line ID 729B04
Locus At1g53320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19893410
Primers used for PCR CR58 - 8474, show primer details
   
Sequence Name 05-K107640-0022-729-B04C-CR58
GenBank Accession LN488383 [GenBank]
Sequencing primer gene specific primer, CR58: 5'-ATGGTAAAATCAATACAAAAGGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K107640-0022-729-B04C-CR58
GAAGTATTTTGCCGAGTTAGAGAGGATCTAGCGAAATCGTGAGTGATTTCTCTCCATTTC
TTAGAAACGCAAGCGCAAGTAACTACATCACGACGTTGAGGCCAACGGTCCTCAGTCTCC
TCCACGCGACGAATGATCTCGCCTAATAATTCAGGGAGCATCGCCGACCATGACGAAGAA
CCGGCCATATTCGACGGCGGAGGAATCGATTCGGATTCCGGTGCCGTCGTTGTCTCTCCC
TGATGAAACCTAAAAGAGTTCGAGATCCTCCGCGAAAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 21-K107641-0022-729-B04C-8409
GenBank Accession LN495690 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K107641-0022-729-B04C-8409
ATTTACATTGAATTATCCTTTCGCGGAGGATCTCGAACTCTTTTAGGTTTCATCAGGGAG
AGACAACGACGGCACCGGAATCCGAATCGATTCCTCCGCCGTCGAATATGGCCGGTTCTT
CGTCATGGTCGGCGATGCTCCCTGAATTATTAGGCGAGATCATTCGTCGCGTGGAGGAGA
CTGAGGACCGTTGGCCTCAACGTCGTGATGTAGTTACTTGCGCTTGCGTTTCTAAGAAAT
GGAGAGAAATCACTCACGATTTCGCTAGATCCTCTCTTAACTCTGGCAAAATTACTTTCC
CTTCTTGCCTCAAATTGGTACGCCTTTTGTATTGATTTTACC

Last Updated on 06.12.2018 16:11