Sequences from confirmation amplicon


Confirmation sequences for line 729H06 and hit At5g26050 are shown.
Line ID 729H06
Locus At5g26050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9103483
Primers used for PCR CR59 - 8474, show primer details
   
Sequence Name 09-K105749w-0022-729-H06C-CR59
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, CR59: 5'-TCAAGAACTAAGAAAACATTGCGA-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K105749w-0022-729-H06C-CR59
CGGCTGGTGCTTCTTCGTGGCGACGGTTTTGTTAGCAGCGGTGTTGTTACCCGCACCAAC
CATAGCGGGTCGACTAGAGCTAAAGAATGAAATCTCGGGGGTCGCTAGTGTCCGTAAAGC
GAAGCTAGCCGTGCGGTGCTGGTCAAACGAGGATGACCTCGGGTGGAATATGCTAAAGCC
GAAGCAATCGCGGATATGGAAGTTCACGACAATGAACATGTGGCCCTTTCAGAAAACCGA
GTTCCGTTGCCAGTTTCGTAGCGGGTTTGGAACCACGAACGAAGACGTGGTGACAGTGTT
TTCTGTCAAGGACGGGTTTCGAAAGCAATGCGGGGTGGGAGGGGACGAGTGCTTTTGGGT
AGCGAAGAGAGATGGGTTCTACATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 49-K105750-0022-729-H06C-8409
GenBank Accession LN506844 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K105750-0022-729-H06C-8409
TTACATTGAATATATGTAGAACCCATCTCTCTTCGCTACCCAAAAGCACTCGTCCCCTCC
CACCCCGCATTGCTTTCGAAACCCGTCCTTGACAGAAAACACTGTCACCACGTCTTCGTT
CGTGGTTCCAAACCCGCTACGAAACTGGCAACGGAACTCGGTTTTCTGAAAGGGCCACAT
GTTCATTGTCGTGAACTTCCATATCCGCGATTGCTTCGGCTTTAGCATATTCCACCCGAG
GTCATCCTCGTTTGACCAGCACCGCACGGCTAGCTTCGCTTTACGGACACTAGCGACCCC
CGAGATTTCATTCTTTAGCTCTAGTCGACCCGCTATGGTTGGTGCGGGTAACAACACCGC
TGCTAACAAAACCGTCGCCACGAAGAAGCACCAGCCGAGGTCTCTTCCCATTTCGATCAA
ATGATCGCAATGTTTTCTTAGGTTCTTGA

Last Updated on 06.12.2018 16:11