Sequences from confirmation amplicon


Confirmation sequences for line 733D11 and hit At1g53840 are shown.
Line ID 733D11
Locus At1g53840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20101577
Primers used for PCR 12cc - 8474, show primer details
   
Sequence Name 50-K108225-0022-733-D11C-12cc
GenBank Accession LN507192 [GenBank]
Sequencing primer gene specific primer, 12cc: 5'-CTCTCCAATGTGTATGCTTGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K108225-0022-733-D11C-12cc
GATGTTCGCTTTCTATGGACCTACTACGGATGTAGACGACTTTGACATATACTTAATACA
TAAAACCATTTTTTGTCTTTAAACCAAATTACAATATAATCAAGTTGATTAATCAAAATA
ATTACAACGTTATCATGAATCTTCACATGCAAGATCCTACACACACACTTAGACGACAAG
AAGTCACCGTGGAACAAGCGCGTCACGTTCATCATTCACATTTACTTAATCCCACTTAAA
AAGGCAAACAACAACATGTCTTCTCTAACATAAACAACAGAACATAACAAAGCCCAAACA
ATCTTAGTAATAATCCCAAAAACCAGAAAAAAAGGAGCTTTGCTTTTGTAGGAATTAATG
GATTCAGTGAACTCCTTCAAAGGATATGGAAAAGTAGACGAAATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 34-K108224-0022-733-D11C-8409
GenBank Accession LN501444 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K108224-0022-733-D11C-8409
GCCATTTACAATTGAATATTTCGTCTACTTTTCCATATCCTTTGAAGGAGTTCACTGAAT
CCATTAATTCCTACAAAAGCAAAGCTCCTTTTTTTCTGGTTTTTGGGATTATTACTAAGA
TTGTTTGGGCTTTGTTATGTTCTGTTGTTTATGTTAGAGAAGACATGTTGTTGTTTGCCT
TTTTAAGTGGGATTAAGTAAATGTGAATGATGAACGTGACGCGCTTGTTCCACGGTGACT
TCTTGTCGTCTAAGTGTGTGTGTAGGATCTTGCATGTGAAGATTCATGATAACGTTGTAA
TTATTTTGATTAATCAACTTGATTATATTGTAATTTGGTTTAAAGACAAAAAATGGTTTT
ATGTATTATGTATATGTCAAAGTCGTCTACATCCGTAGTAGGTCCATAGAAAGCGAACAT
TCTTTACAATTCTATGGGTTCCTCGGTATATTTTTCAAGCATACACATTGGAGAG

Last Updated on 06.12.2018 16:11