Sequences from confirmation amplicon


Confirmation sequences for line 734B03 and hit At2g45310 are shown.
Line ID 734B03
Locus At2g45310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18683795
Primers used for PCR 67bi - 8474, show primer details
   
Sequence Name 44-K114805-0022-734-B03C-67bi
GenBank Accession LN505278 [GenBank]
Sequencing primer gene specific primer, 67bi: 5'-TATAGTCTGGGCTTCGTCTAGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K114805-0022-734-B03C-67bi
AATAACCGGCGTGTTTATACGCCGCCACTAAAAAAGCCGGCGAAGAGATCGCACATACCT
ACAACCACATCTATGGATTATCTCTAACCGGATTAAGATTTTTCACCGTGTATGGACCTT
GGGGAAGACCAGACATGGCGTATTTCTTCTTCACTAAAGATATTCTAAAAGGCAAATCGA
TTTCGATATTCGAATCGGCGAATCACGGAACGGTGGCTAGGGATTTCACTTACATAGACG
ATATAGTGAAGGGATGTTTAGCGGCGCTAGATACGGCGGAGAAGAGTACAGGGAGTGGAG
GGAAGAAGAGAGGTCCGGCGCAATTGAGAGTGTTTAACCTCGGAAACACGTCGCCGGTGC
CGGTTTCAGATCTGGTGAGGATATTGGAGAGACAATTGAAAGTGAAGGCGAAGAAGAATT
TGATTAAGATGCCACGAAACGGTGACGTTCCGTTTACTCATGCTAATATNAGCAATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATT
   
Sequence Name 20-K114804-0022-734-B03C-8409
GenBank Accession LN495327 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K114804-0022-734-B03C-8409
AATTCTTCTTCGCCTTCACTTTCAATTGTCTCTCCAATATCCTCACCAGATCTGAAACCG
GCACCGGCGACGTGTTTCCGAGGTTAAACACTCTCAATTGCGCCGGACCTCTCTTCTTCC
CTCCACTCCCTGTACTCTTCTCCGCCGTATCTAGCGCCGCTAAACATCCCTTCACTATAT
CGTCTATGTAAGTGAAATCCCTAGCCACCGTTCCGTGATTCGCCGATTCGAATATCGAAA
TCGATTTGCCTTTTAGAATATCTTTAGTGAAGAAGAAATACGCCATGTCTGGTCTTCCCC
AAGGTCCATACACGGTGAAAAATCTTAATCCGGTTAGAGATAATCCATAGATGTGGTTGT
AGGTATGTGCGATCTCTTCGCCGGCTTTTTTAGTGGCGGCGTATAAACTCGCCGGTTGAT
CTGTTTTATCTTTCTCCGAGAACGGAACTTTGGTGTTGAGTCCGTAGA

Last Updated on 06.12.2018 16:11