Sequences from confirmation amplicon


Confirmation sequences for line 735E12 and hit At3g50220 are shown.
Line ID 735E12
Locus At3g50220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18618398
Primers used for PCR MG11 - 8474, show primer details
   
Sequence Name 17-K031456-022-735-E12C-8409
GenBank Accession LN493736 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K031456-022-735-E12C-8409
CATTTACAATTGAATATATCCTGATTAACAATCAACGCTCCAACTTACCAACTAGTGCAA
TCAACGCTTTGCTTCACTACGCGTCAAGATCCAACGATAGTTTCCACATGTCTTACGGAG
AGATGAAATCAATCTCTGACGTCCTCCGCCGCTGCGCTCCACCGTGTAATCTTCTCGTTT
TCGGACTCACACACGAGACTCTCCTTTGGAAGTCGTTAAACCACAACGGTAGAACAGTGT
TCATCGAGGAGAATCGTTACTACGCGGCTTACTTCGAAGAAATCCACCCGGAGATCGACG
TTTTCGACGTTCAATACACGACAAAAGCTCACGAAGCTGGAGAGCTCGTCACCGCGGCTA
AAGAAGCCGCGGGAAACGAGTGTCGTCCTGTGCAAAATCTTCTCTTCTCAGATTGTAAAT
TAGGTCTCAATGATTTGCCTAATCATGTCTCGGATGTTGA
   
Sequence Name 21-K031456-022-735-E12C-MG11
GenBank Accession LN495550 [GenBank]
Sequencing primer gene specific primer, MG11: 5'-TCAACATCGTAGACATGATTAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K031456-022-735-E12C-MG11
TCTGAGAGAGAGATTTTGCACAGGACGACACTCGTTTCCCGCGGCTTCTTTAGCCGCGGT
GACGAGCTCTCCAGCTTCGTGAGCTTTTGTCGTGTATTGAACGTCGAAAACGTCGATCTC
CGGGTGGATTTCTTCGAAGTAAGCCGCGTAGTAACGATTCTCCTCGATGAACACTGTTCT
ACCGTTGTGGTTTAACGACTTCCAAAGGAGAGTCTCGTGTGTGAGTCCGAAAACGAGAAG
ATTACACGGTGGAGCGCAGCGGCGGAGGACGTCAGAGATTGATTTCATCTCTCCGTAAGA
CATGTGGAAACTATCGTTGGATCTTGACGCGTAGTGAAGCAAAGCGTTGATTGCACTAGT
TGGTAAGTTGGAGCGTTGATTGTTAATCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11