Sequences from confirmation amplicon


Confirmation sequences for line 736B11 and hit At3g29380 are shown.
Line ID 736B11
Locus At3g29380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:11282548
Primers used for PCR TL01 - 8474, show primer details
   
Sequence Name 27-K026966-022-736-B11C-8409
GenBank Accession LN498231 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K026966-022-736-B11C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATACGATCAAGAACTCGGTTAAAGA
TATGTACCCGTACGCCTTGAAGATTATACCTAACTGGTATGCTTGTGAATCAGACATCAT
CAAAAGGTTAGATGGTGTCATCACGTCCTGGGATTCTGCAAAATTTTCAGTATAAGGAAG
TCACTACCTGTGAAAAGAAGAAGCTTTGGGATTGTTGTAGTCTCGTTTCTGATGCATTAC
ATACATATATGTAAAGTCGTTGGCTTTATGGAAGTATATTTTAGACATCTTATGTAAAAT
CTTCTTTATGATATACTGCATTTTATGGTATGGGCCGAATAGACATCAAATAAATTCAGA
GTAAACAATGTGGGAAAACAAAATCT
   
Sequence Name 28-K026966-022-736-B11C-TL01
GenBank Accession LN498696 [GenBank]
Sequencing primer gene specific primer, TL01: 5'-GAGATTTTGTTTTCCCACATTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K026966-022-736-B11C-TL01
TCTGAATTTATTTGATGTCTATTCGGCCCATACCATAAAATGCAGTATATCATAAAGAAG
ATTTTACATAAGATGTCTAAAATATACTTCCATAAAGCCAACGACTTTACATATATGTAT
GTAATGCATCAGAAACGAGACTACAACAATCCCAAAGCTTCTTCTTTTCACAGGTAGTGA
CTTCCTTATACTGAAAATTTTGCAGAATCCCAGGACGTGATGACACCATCTAACCTTTTG
ATGATGTCTGATTCACAAGCATACCAGTTAGGTATAATCTTCAAGGCGTACGGGTACATA
TCTTTAACCGAGTTCTTGATCGTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11