Sequences from confirmation amplicon


Confirmation sequences for line 736G01 and hit At5g50130 are shown.
Line ID 736G01
Locus At5g50130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20391016
Primers used for PCR NL53 - 8474, show primer details
   
Sequence Name 04-K103931w-0022-736-G01C-NL53
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, NL53: 5'-CAACGGAAGATAGAGAGCTCAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K103931w-0022-736-G01C-NL53
TGTAGCTTCTGGATTCCTCTTATTATTCTCTCCTTCACCATTTCTGCTTTCTTCATATCT
CTTACTGCCATCACTACTCTCACCCCTCTTTTTGCTAACACTCTCGCAGTTTCCGCACCG
ATCCCAGACGTTCCTCCTGTTAGAAAGTAGTATGCATCAAACATTTCATAAAAAAATACA
ATCTAGTATATAAAAAACATGAACCATTTCACTTCGTATCAGTTAATTTTGAAATGGTGC
TTTTGGACTTTCTTAGATAAAAATAATTTTAGCAAGTGTATTTAACAAAAGTGAAACTTA
TTTGTTATTAAGTATTTTCTCCAAATTATGTGTGTATATGGAAAAAATAAAAAAGAGTCC
ACAGTTAAATTCAGATCCAGCATAACTGAAATCATAATTAACCAGATTATTTCACTACAA
TTGGGTAGTGGAATTGGTTTTCGACAATTGATGTCTCTAAAAGCAGCCTTTTTGTTTTTC
TTTGCAACATAAAGAGAGAGTAAAGAGAGGCCACTTTAAAATTCATTGACCCTCATTGCA
TAAAGTTTTAACCGACAAATTAGGGAACATAAAGATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATT
   
Sequence Name 20-K103932w-0022-736-G01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K103932w-0022-736-G01C-8409
TCTATTTGCGGTAAACTTTATGAATGAGGGTCAATGAATTTTAAAGTGGCCTCTCTTTAC
TCTCTCTTTATGTTGCAAAGAAAAACAAAAAGGCTGCTTTTAGAGACATCAATTGTCGAA
AACCAATTCCACTACCCAATTGTAGTGAAATAATCTGGTTAATTATGATTTCAGTTATGC
TGGATCTGAATTTAACTGTGGACTCTTTTTTATTTTTTCCATATACACACATAATTTGGA
GAAAATACTTAATAACAAATAAGTTTCACTTTTGTTAAATACACTTGCTAAAATTATTTT
TATCTAAGAAAGTCCAAAAGCACCATTTCAAAATTAACTGATACGAAGTGAAATGGTTCA
TGTTTTTTATATACTAGATTGTATTTTTTTATGAAATGTTTGATGCATACTACTTTCTAA
CAGGAGGAACGTCTGGGATCGGTGCGGAAACTGCGAGAGTGTTAGCAAAAAGAGGGGTGA
GAGTAGTGATGGCAGTAAGAGATATGAAGAAAGCAGAAATGGTGAAGGAGAGAATAATAA
GAGAGAATCCAGAAGCTGACATTATATTATTCGAGATTGATCTGAGCTCTCTATCTTCCG
T

Last Updated on 06.12.2018 16:11