Sequences from confirmation amplicon


Confirmation sequences for line 737G06 and hit At5g45480 are shown.
Line ID 737G06
Locus At5g45480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18427775
Primers used for PCR 15xq - 8474, show primer details
   
Sequence Name 50-K119444-0022-737-G06C-15xq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 15xq: 5'-ATCCATTTCCATCACATAGCGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K119444-0022-737-G06C-15xq
GTCGCGCAGTGTCCAGTCACCTCTTGCTGAAGATACCCTCATGGCGCTTTCAGAGTCGTC
AGCGAAACGGGATTTGGCTTTAACCTCTCCGAATATGTGTTCCCACATATCCAGGGTGAG
ACGATCACTAGATGTGTAGATCACCTCATCTAGCTGAGCTCGGATGCCAAAGAACTCCAT
AAAACTTTTTATCCAGCGTCCAGGTAGACTAGGAATGTAAAATATGCAGAAGAAGCTAAA
CCTAATGTACTCTTCATTCAACTTCGGGTATTTCTTGCATAAACGACTTACTTTACCGTC
AATCCACTTGAAAGTCTGATGAATTCTCTGTTTTGTGACTCTAAAACAGGATTCAATGAC
ACTTCCAAGTTTTGTGAATGCGGGGCCAATGTAAAGTATGTTGATAACCTTGTCAAAGGC
GATGTGTATGAAACCCTTGGTATGGTGGATTCGCTTCGGTTTTAAACCTAGGCAGTATTC
GATCAGGTTGTGTGCATGTACATACTCAGACCACCGGCGATACATGAAACTCGTGTCGAG
CACTTTATGACATGGTATTGTCTTGGCACTCTTGTCGGGCACTTTCCGAGACCTGGTATT
TTGCTGGTACTCTTGTCGAGAACTTCATGAGACTTCTCTGTCTTCAACTCCCTCACCCTG
AGAATCCTGTTAAGAATCCTTTTAAGAAATGGTGTTTTCTTAGTACTCGTGTTGGGCACT
TCATGAGACTGTGTATTCTTGTAGCATTCATCATACTCCTCCACCTTCAATTTCCTGACC
CCGAGAATGTTTCCCGGATATATTCAATTGTAAATGGCTTCATGTCCGGGGAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATT
   
Sequence Name 18-K119443-0022-737-G06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K119443-0022-737-G06C-8409
ATTTACATTGAATATATCCTGGAAACATTCTCGGGGTCAGGAAATTGAAGGTGGAGGAGT
ATGATGAATGCTACAAGAATACACAGTCTCATGAAGTGCCCAACACGAGTACTAAGAAAA
CACCATTTCTTAAAAGGATTCTTAACAGGATTCTCAGGGTGAGGGAGTTGAAGACAGAGA
AGTCTCATGAAGTTCTCGACAAGAGTACCAGCAAAATACCAGGTCAGGAAGTGCCCGACA
AGAGTGCCAAGACAATACCATGTCATAAAGTGCTCGACACGAGTTTCATGTATCGCCGGT
GGTCTGAGTATGTACATGCACACAACCTGATCGAATACTGCCTAGGTTTAAAACCGAAGC
GAATCCACCATACCAAGGGTTTCATACACATCGCCTTTGACAAGGTTATCAACATACTTT
ACATTGGCCCCGCATTCACAAAACTTGGAAGTGTCATTGAATCCTGTTTTAGAGTCACAA
AACAGAGAATTCATCAGACTTTCAAGTGGATTGACGGTAAAGTAAGTCGTTTATGCAAGA
AATACCCGAAGTTGAATGAAGAGTACATTAGGTTTAGCTTCTTCTGCATATTTTACATTC
CTAGTCTACCTGGACGCTGGATAAAAAGTTTTATGGAGTTCTTTGGCATCCGAGCTCAGC
TAGATGAGGTGATCTACACATCTAGTGATCGTCTCACCCTGGATATGTGGGAACACATAT
TCGGAGAGGTTAAAGCCAAATCCCGTTTCGCTGACGACTCTGNAAGCGCCATGAGGGTAT
CTTCAGCAAGAGGTGACTGGACACTGCGCGACATACAAGGTGACCCAGAAACAGAANAAA
AACGTGAGAAACTTCTACGCTATGT

Last Updated on 06.12.2018 16:11