Sequences from confirmation amplicon


Confirmation sequences for line 741H03 and hit At1g73660 are shown.
Line ID 741H03
Locus At1g73660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27696305
Primers used for PCR 49vf - 8474, show primer details
   
Sequence Name 31-K117753-0022-741-H03C-49vf
GenBank Accession LN500326 [GenBank]
Sequencing primer gene specific primer, 49vf: 5'-AAGTGGCAAAACCATGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K117753-0022-741-H03C-49vf
AGCTCTCAACATGCTCTCTGGGTGCACGACTGGTCCACCCATGTAATCTCCCACTAAAAT
AGCCAGTTTCCTTACCAATTCACTATTCACAAAACCAGAGGAAGAAACTGATCTTGATTT
AGCAGCAATATCAAGAGCCATCTGTTCAAGTCTCAACAGATTAGAATCCCCACTTCTGTT
CACAAGAACAGCTTCCCATGTCACACCGTCTGAAACAGGTGTCCCTTGAAGATCGAGCAA
AGGAGGTATTCTTTCTGCTGAGGATGCATTCAACACTCCATACAAGTCATAAAAACCATC
CAAGATCTTGTCATCATAGCCAAGACAATTGTAATTCTGTGAATGCAACAATACCAAAAT
GAAATGAGTTCTTGGGAGTAACTAAAGAGTTTCTGTTATGCAGAGAAAACAATTGAATCA
AAAGCTTACCCAGTAGCGATAAGCGATGAGTTCAGCTGGAGAGTTCTCAGGAGCACAAGA
GCCTAAACTGAATTGCTTAATAGCCTCAATCTGAGTAGCCTCAGGATCTTCTCTAGCACT
TAACTCTAAAGCCAACTGTATCTGATTCTCTTCTTCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTC
   
Sequence Name 15-K117752-0022-741-H03C-8409
GenBank Accession LN493159 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K117752-0022-741-H03C-8409
TTTACATTGAATATATCCTGAAGAAGAGAATCAGATACAGTTGGCTTTAGAGTTAAGTGC
TAGAGAAGATCCTGAGGCTACTCAGATTGAGGCTATTAAGCAATTCAGTTTAGGCTCTTG
TGCTCCTGAGAACTCTCCAGCTGAACTCATCGCTTATCGCTACTGGGTAAGCTTTTGATT
CAATTGTTTTCTCTGCATAACAGAAACTCTTTAGTTACTCCCAAGAACTCATTTCATTTT
GGTATTGTTGCATTCACAGAATTACAATTGTCTTGGCTATGATGACAAGATCTTGGATGG
TTTTTATGACTTGTATGGAGTGTTGAATGCATCCTCAGCAGAAAGAATACCTCCTTTGCT
CGATCTTCAAGGGACACCTGTTTCAGACGGTGTGACATGGGAAGCTGTTCTTGTGAACAG
AAGTGGGGATTCTAATCTGTTGAGACTTGAACAGATGGCTCTTGATATTGCTGCTAAATC
AAGATCAGTTTCTTCCTCTGGTTTTGTGAATAGTGAATTGGTAAGGAAACTGGCTATTTT
AGTGGGAGATTACATGGGTGGACCAGTCGTGCACCCAGAGAGCATGTTGAGAGCTTGGAG
GAGTCTTAGCTATAGTTTGAAAGCAACTCTTGGAAGCAT

Last Updated on 06.12.2018 16:11