Genotyping and primer details


Genotyping and primer details for 741H03 - At1g73660
Line ID 741H03
Locus At1g73660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27696305 of line 741H03 Chr1:27696305
   
Insertion allele amplicon 8474 - 49vf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 49vf: 5'-AAGTGGCAAAACCATGCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27695672
   
wt amplicon (over InsPos) 23vp - 49vf
wt amplicon start Chr1:27695672
wt amplicon end Chr1:27696926
wt amplicon size 1255 bp
Locus specific primer 49vf: 5'-AAGTGGCAAAACCATGCTT-3'
2nd locus specific primer 23vp: 5'-GTGCTTTTAACCATTTTGGTGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11