Sequences from confirmation amplicon


Confirmation sequences for line 742A03 and hit At5g39220 are shown.
Line ID 742A03
Locus At5g39220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15706919
Primers used for PCR 33sf - 8474, show primer details
   
Sequence Name 44-K116518-0022-742-A03C-33sf
GenBank Accession LN505307 [GenBank]
Sequencing primer gene specific primer, 33sf: 5'-GGAATTCTTTTGGGCTTTTAGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K116518-0022-742-A03C-33sf
CAGCTTGTCTTGAGACTTGGGCTATTGATGTTCTTGGTTGGGGATTCTCAGATCTTGGTA
AGATTTTACATTTTACACAAATTAAACAAGATTCTTGATAATTCGATTTTAGTACATATG
AATGTGTGATTGTTTCTTTTCACAACTTAATGACACTGTAGAGAAACTTCCACCATGTGA
TGCAGCATCTAAGCGACATCATTTATTCGAGGTCAGTACTATTTATTAATTATACTACAA
GAAAACAAGCGAATAACAACGAAAAACTGTGTCACTAAATGAATGAAATCAGTCGATGAA
TCATATTCTTTTGGCACAAATAACTATACACTAAATGGACTATTATCGACGAATACAAAT
TCGTGTCGTCGCTAAATAACAACAGATACAATGTTGTGGCAAATTTAGCATTGATGTTAG
AACTCTCTAGTCCGTCTTGAATTTCCTGATGATTTTGCGATATATTTTGTTGGTTGGTAA
CTAAAAATTGATACAATTATTTTGCGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTATATGT
   
Sequence Name 18-K116517-0022-742-A03C-8409
GenBank Accession LN494466 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K116517-0022-742-A03C-8409
GCCATTTACATTGAATATATCGCAAAATAATTGTATCAATTTTTAGTTACCAACCAACAA
AATATATCGCAAAATCATCAGGAAATTCAAGACGGACTAGAGAGTTCTAACATCAATGCT
AAATTTGCCACAACATTGTATCTGTTGTTATTTAGCGACGACACGAATTTGTATTCGTCG
ATAATAGTCCATTTAGTGTATAGTTATTTGTGCCAAAAGAATATGATTCATCGACTGATT
TCATTCATTTAGTGACACAGTTTTTCGTTGTTATTCGCTTGTTTTCTTGTAGTATAATTA
ATAAATAGTACTGACCTCGAATAAATGATGTCGCTTAGATGCTGCATCACATGGTGGAAG
TTTCTCTACAGTGTCATTAAGTTGTGAAAAGAAACAATCACACATTCATATGTACTAAAA
TCGAATTATCAAGAATCTTGTTTAATTTGTGTAAAATGTAAAATCTTACCAAGATCTGAG
AATCCCCAACCAAGAACATCAATAGCCCAAGTCTCAAGACAAGCTTGTTCAAGCAGAGGA
TAAGTACGTCTCCATTCAAGACAAGAACTAAAAGCCCAAAAGAAATTC

Last Updated on 06.12.2018 16:11