Sequences from confirmation amplicon


Confirmation sequences for line 742B08 and hit At5g18500 are shown.
Line ID 742B08
Locus At5g18500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6139802
Primers used for PCR CR71 - 8474, show primer details
   
Sequence Name 39-K107114-0022-742-B08C-8409
GenBank Accession LN503371 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K107114-0022-742-B08C-8409
GCCTTTACAATTGAATATATCCTGACATGAACAACTCCATATCCACCATCACCGATGATA
TTATCCCTTGAAAACTGATTAGTAGCCATCTGAAGATCTCTAAGAGTGAACCAATGTCCC
CATCCAAGGTGAGAAAACTCAGGAAGACCAGACAAAGGAGATGGAGCTGTCAAAGGATTA
GCAGAAGATACGCTCGATCCGTCTTTTTTCTCCAAGTGATTAAACGAGCCTGACCGGCTA
CTATCGCCATTTTCTGACTCTGCTTTTATCCCTTTTTCGGGTTCTTTATCGCCAAATTTC
TCACTAATAGAGGGGTATCCATTCCCACCATTGCTTGAAGAAACCTCGTCGACTCTAATC
TCTTTAATCTCTTCAGGAACACTAGGAGGAATCTGGCGACTAACAGGGATTAGATTAGAA
GAAGATCTAGAGGTTTTTCTTCTGAAAGTAAGCCATAGCGATATAGCTAAAACTACAACG
AATATCGCGGAGAGAACAATCACTATTATCTCCCATAGCTCCA
   
Sequence Name 55-K107115-0022-742-B08C-CR71
GenBank Accession LN508829 [GenBank]
Sequencing primer gene specific primer, CR71: 5'-AACTACAATGGTTTGGAGCTATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K107115-0022-742-B08C-CR71
GTTGTAGTTTAGCTATATCGCTATGGCTTACTTTCAGAAGAAAAACCTCTAGATCTTCTT
CTAATCTAATCCCTGTTAGTCGCCAGATTCCTCCTAGTGTTCCTGAAGAGATTAAAGAGA
TTAGAGTCGACGAGGTTTCTTCAAGCAATGGTGGGAATGGATACCCCTCTATTAGTGAGA
AATTTGGCGATAAAGAACCCGAAAAAGGGATAAAAGCAGAGTCAGAAAATGGCGATAGTA
GCCGGTCAGGCTCGTTTAATCACTTGGAGAAAAAAGACGGATCGAGCGTATCTTCTGCTA
ATCCTTTGACAGCTCCATCTCCTTTGTCTGGTCTTCCTGAGTTTTCTCACCTTGGATGGG
GACATTGGTTCACTCTTAGAGATCTTCAGATGGCTACTAATCAGTTTTCAAGGGATAATA
TCATCGGTGATGGTGGATATGGAGTTGTTCATGTCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11