Sequences from confirmation amplicon


Confirmation sequences for line 743G04 and hit At5g26130 are shown.
Line ID 743G04
Locus At5g26130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9128523
Primers used for PCR 22xq - 8474, show primer details
   
Sequence Name 33-K119356-0022-743-G04C-22xq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 22xq: 5'-GCCTAGTATATTTATTCCGGGCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K119356-0022-743-G04C-22xq
CTTTGAATCTCTGAATAACACTAATGTTTTACCAAATTACTTTAGTATTATCCATAGATT
ATATCTCAGAAATGGATCCAACGAGAAAACAAAGAAAAATTGTGGAAGTCCCCTTTAGTG
GTTAACTAAACTTATATATATGGAGTGTAATTTCACGAAACTAAATCGCAGAAACTAAAT
TATGAAGTCTTATTTTTATCTAAATATGGAAGTCCTCTTACTATGACAGGTCAGATGAAT
TAGTTATTGATATTACGTTACGTTAGACACACGTTTCAGCGTATACGATTCATCTAAAAC
TTTAGACAGTGATCCGGCGATTTGATGGTCCGATGATAATAGAACAAAAGGAATTGGACG
ATGACAATAGACAGTCTCCAACAAGTGTCAATGTGGGATTTTCTTTCATTGTTCCGATGA
AAAAGCCAAATTTAACAGTGTAATGTAAGCATGAACTACGAAGAGTAAGAGTATGCCGAT
GGAATATGTCTATATGATATGAGAGCTAGTATATATATCGTTCTAATATATGGAAATTTA
AGTGTGAAAGGGATACATCTTTAAGAGGTAGAACTCATATCAGGTTAAATTCATCTCTTT
TAAAGAAGATATTTTTTCAAACAGACGTCGAACACAATACTTTCAATTGGTGTATATTTT
CTATTTAAACTTTAGTATTTTTTTATAACAAATAGTGTTTTACTATTATTTGTTTTGCTT
TATAGTTATCCGGATGTTTTCAAATCGAAATGAAACGGATAATAGATCAAGTAAATCTAG
TGGATATTTAGTCTAATCCTAATGAAAATATGCTTGATTTGTTTAGTGACTCGACATCCG
TTCTTACGTACTCTGTACTTCTTCCTATGAGACTAACTTNAACAATGGCAAATGGTTGAT
TCTTAAAGGGAAGATAATTAATCTTTGATCATGTCTCATTGCTAGCTAGGATCTCTGACC
CCTTCCCTGATTGAC
   
Sequence Name 13-K119355-0022-743-G04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K119355-0022-743-G04C-8409
GGTCTTGGGCTTTCGAGGAACTGCAAAAGCTAGGAAAAGAACTATAACCGAAAGTATTAG
TGTTTGAGGAGATTTAAACATCTTCATTCTTTTTCCTTATTAGTTGTCTATGAGCGAGCT
TGTGTTGTATGTATTGGGTTAGAAACGGGGATTTATATAGAGTTTACGTTGGAGTTATTT
GATGAAAGATTAATAACACTTGTAGTAATTTTATCATTTAAAAAAAAAAACTGTTTTAAC
CAAGTCAATCAGGGGAAGGGGTCAAAGATCCTAGCTAGCAATGAGACATGATCAAAGATT
AATTATCTTCCCTTTAAAAATCAACCATTTGCCATTGTTTAAGTTAGTCTCATAGGAAGA
AGTACAGAGTACGTAAAAACGGATGTCGAGTCACTAAACAAATCAAGCATATTTTCATTA
GGATTAGACTAAATATCCACTAGATTTACTTGATCTATTATCCGTTTCATTTCGATTTGA
AAACATCCGGATAACTATAAAGCAAAACAAATAATAGTAAAACACTATTTGTTATAAAAA
AATACTAAAGTTTAAATAGAAAATATACCCCATTTGAAAGTATTGTGTTCGACGTCTGTT
TGAAAAAATATCTTCTTTAAAAGAGATGAATTTAACCTGATATGAGTTCTACCTCTTAAA
GATGTATCCCTTTCACACTTAAATTTCCATATATTAGAACGATATATATACTAGCTCTCA
TATCATATAGACATATTCCATCGGCATACTCTTACTCTTCGAAGTTCATGCTTACATTAC
ACTGTTAAATTTGGCTTTTTCATCGGAACAATGAAAGAAAATCCCACATTGACACTTGTT
GGAGACTGTCTATGGTCATC

Last Updated on 06.12.2018 16:11