Sequences from confirmation amplicon


Confirmation sequences for line 745A07 and hit At2g03505 are shown.
Line ID 745A07
Locus At2g03505 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1064828
Primers used for PCR CR76 - 8474, show primer details
   
Sequence Name 63-K107248-0022-745-A07C-CR76
GenBank Accession LN511002 [GenBank]
Sequencing primer gene specific primer, CR76: 5'-CTTGTTTCTCTTGGCTTTAACCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K107248-0022-745-A07C-CR76
TTTGTCAATGATTCTCTGTTCTTGAGGTTTCAAACAAAACCAACTCTCTCTCACTTTCTC
TGTTTCTAGGTGCAACTTATTGTCTATGTAGAGATGGTGTTGGAGAGAAAGATCTTCAAA
CATCGATAGATTATGCATGTGGAGTTTTAAAAGATTGTAATCCTATTCATGAGAAAGGTC
CTTGTTATCAACCAAACACCATCAAGAGTCATTGTGATTGGGCGGTTAATACTTATTTCC
AAAGATTTGGTCAAATCTCTGGAAGCTGTAATTTCTCAGGAACTGCTACTACCAGCCAAA
ATCTTCCTTCAAGTAAAAACCAAAATCTCTGAAACCTAAATTTGTTGGTGTTATGATGCA
ATCTTTTTGATGTTTTCCTCTGTTCTTTTGCAGCTGTGGTTACTGGTTGCTTATATCCTT
CAAGTCCAGGGTATGTTTGTTTCAAGATTTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 47-K107247-0022-745-A07C-8409
GenBank Accession LN506212 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K107247-0022-745-A07C-8409
CGGACTGAGCCATTTAATCTTGAAACAAACATACCCTGGACTTGAAGGATATAAGCAACC
AGTAACCACAGCTGCAAAAGAACAGAGGAAAACATCAAAAAGATTGCATCATAACACCAA
CAAATTTAGGTTTCAGAGATTTTGGTTTTTACTTGAAGGAAGATTTTGGCTGGTAGTAGC
AGTTCCTGAGAAATTACAGCTTCCAGAGATTTGACCAAATCTTTGGAAATAAGTATTAAC
CGCCCAATCACAATGACTCTTGATGGTGTTTGGTTGATAACAAGGACCTTTCTCATGAAT
AGGATTACAATCTTTTAAAACTCCACATGCATAATCTATCGATGTTTGAAGATCTTTCTC
TCCAACACCATCTCTACATAGACAATAAGTTGCACCTAGAAACAGAGAAAGTGAGAGAGA
GTTGGTTTTGTTTGAAACCTCAAGAACAGAGAATCATTTTGAACAAAAAGCTTACTTGAG
TAGGTGGTTAAAGCCAAGAAAAACAA

Last Updated on 06.12.2018 16:11