Genotyping and primer details


Genotyping and primer details for 745A07 - At2g03505
Line ID 745A07
Locus At2g03505 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:1064828 of line 745A07 Chr2:1064828
   
Insertion allele amplicon 8474 - CR76
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CR76: 5'-CTTGTTTCTCTTGGCTTTAACCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:1064337
   
wt amplicon (over InsPos) 49wn - CR76
wt amplicon start Chr2:1064337
wt amplicon end Chr2:1065394
wt amplicon size 1058 bp
Locus specific primer CR76: 5'-CTTGTTTCTCTTGGCTTTAACCAC-3'
2nd locus specific primer 49wn: 5'-GAAATAAAACTTTTGCCTCACCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11