Sequences from confirmation amplicon


Confirmation sequences for line 745D06 and hit At5g55470 are shown.
Line ID 745D06
Locus At5g55470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22471734
Primers used for PCR EP40 - 8474, show primer details
   
Sequence Name 23-K103006-0022-745-D06C-8409
GenBank Accession LN496512 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K103006-0022-745-D06C-8409
GCCATTTACATTGAATATATCCTGGAAATCAAAACAAGAAAGCATTCACTCTCGCAAACT
GATAAGTAATAACCATCTCAACAAGAAATGAAAAGAAACATAGATCAGTGAAAAATCTTA
CCAAGATAGTCTCTAGCACTCAACCCCTTAAATCCCAACTTGGGAAACAGCCACCAAGTC
CCTGGTTCACATTATAAAAACAACAAAAATAGAGATGGTAACAACTCCGAGATAATCAAC
TTTAAATCATATAGTCAAGTCATTCAAAGATTAATGAAATATTTTTTACCAAACGAGATA
ATGACAGTGGAGATGAAAACTCCAATCACACCAAAGGACATGATGGTTAAAAAGTTGTGA
AAAAACTTCTTTTTCTTAACTTGGAACCTGCCACAACAAACCAAAAAATCTTCAGTCAAT
CACCATCATAGTGTAATCTAACCCATTAACCACAACATAGTAACCAAAACCATAGTAAGC
TTACCCAGCANTGAAGATTATNGGAGGAAGAAGGTAAATGAAGA
   
Sequence Name 71-K103007-0022-745-D06C-EP40
GenBank Accession LN512540 [GenBank]
Sequencing primer gene specific primer, EP40: 5'-TTCTTCATTTACCTTCTTCCTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K103007-0022-745-D06C-EP40
GGTTTTGGTTACATGTTGTGGTAATGGGTTAGATTACACTATGATGGTGATTGACTGAAG
ATTTTTTGGTTTGTTGTGGCAGGTTCCAAGTTAAGAAAAAGAAGTTTTTTCACAACTTTT
TAACCATCATGTCCTTTGGTGTGATTGGAGTTTTCATCTCCACTGTCATTATCTCGTTTG
GTAAAAAATATTTCATTAATCTTTGAATGACTTGACTATATGATTTAAAGTTGATTATCT
CGGAGTTGTTACCATCTCTNTTTTTGTTGTTTTTATAATGTGAACCAGGGACTTGGTGGC
TGTTTCCCAAGTTGGGATTTAAGGGGTTGAGTGCTAGAGACTATCTNGGTAAGATTTTTC
ACTGATCTATGTTTCTTTTCATTTCTTGTTGAGATGGTTATTACTTATCAGTTTGCGAGA
GTGAATGCTTTCTTGTTTTGATTTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTNTTATAAAATGAAAG
TACATTTTGATAAAACGACNAATTACGATCCGTCGTATTTATNGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATATCGACGTGTCTACGTTCACTC

Last Updated on 06.12.2018 16:11