Sequences from confirmation amplicon


Confirmation sequences for line 746A06 and hit At1g66760 are shown.
Line ID 746A06
Locus At1g66760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24904012
Primers used for PCR 17ow - 8474, show primer details
   
Sequence Name 57-K115005-0022-746-A06C-17ow
GenBank Accession LN509499 [GenBank]
Sequencing primer gene specific primer, 17ow: 5'-TAGTCTGTTCTTGTCTCGCAGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K115005-0022-746-A06C-17ow
GTGACTGATATCACTCCTATTCTTTGTATCTCAATCCTCATGGACAGCTTCTTGACCGTC
CTCTCCGGTTAGCCTTTCTTAACACGCATCTCCGGTTTTGCAACCATGTTATTATTTTTT
TGTGAACGTGAACCGAATATATGTTTTGTTTTGTTTAGGGATTGTGAGAGGAACAGGGTG
GCAAAAAATTGGAGCTTATGTGAACATAACCTCTTATTATGTCATTGGTATTCCTGTTGG
ACTTCTATTGTGTTTTCATCTTCACTTCAATGGGAAAGGACTTTGGGCTGGTTTAGTCAC
AGGATCAACATTACAAACTCTAATCCTATTTCTTGTTATTTCTTGTCACAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCACGNTTATT
   
Sequence Name 25-K115004-0022-746-A06C-8409
GenBank Accession LN497573 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K115004-0022-746-A06C-8409
CATTGAATATATCCTGTGACAGAAATAACAAGAAATAGGATTAGAGTTTGTAATGTTGAT
CCTGTGACTAAACCAGCCCAAAGTCCTTTCCCATTGAAGTGAAGATGAAAACACAATAGA
AGTCCAACAGGAATACCAATGACATAATAAGAGGTTATGTTCACATAAGCTCCAATTTTT
TGCCACCCTGTTCCTCTCACAATCCCTAAACAAAACAAAACATATATTCGGTTCACGTTC
ACAAAAAAATAATAACATGGTTGCAAAACCGGAGATGCGTGTTAAGAAAGGCTAACCGGA
GAGGACGGTCAAGAAGCTGTCCATGAGGATTGAGATACAAAGAATAGGAGTGATATCAGT
CACATAACTAATTACTTCCTCCACATTGCTATATGCATAAGGCCACACACTGCGAGACAA
GAACAGACT

Last Updated on 06.12.2018 16:11