Sequences from confirmation amplicon


Confirmation sequences for line 748E05 and hit At5g16450 are shown.
Line ID 748E05
Locus At5g16450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5374595
Primers used for PCR MC86 - 8474, show primer details
   
Sequence Name 58-K102176-0022-748-E05C-8409
GenBank Accession LN509662 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K102176-0022-748-E05C-8409
CATTTACATTGCAAGTAAAATTTGTTCAAGCCATTGCAATCTTGAACTTGATTTGCAAAT
ACCTTTCTCCTCGATGAATTGACGGATTAAGCCATTGTCTTCAAAAACTTTGACAGTGAC
AACTGGTCCTGAGAATATTTGTCGACGGCCATAAATCTGGAAAATGGGTTGCAAAGCTCG
TAGCTGACCACTCCGAATCAGCTCTTGATTCGCGTCACAAACTTCCGCAGTTGTCACAAA
CGCCATTTTCTTCTTTTTCCTGTTCTCAGCTATGATCAAAACATGAGACTTGCGACTGAA
TCAATCAAAAGTAATCTGAGATCATGATGATATGATATGATCAAACACATAATGAAAATA
ATCAGATTTTCAGATTTTCGATATAGCTTGAGTATGATCACCGAATCTGTTTTCTATTTC
ATGGAGAATCAAACAAACCCAGAAGAAAA
   
Sequence Name 58-K102180-0022-748-E05C-MC86
GenBank Accession LN509663 [GenBank]
Sequencing primer gene specific primer, MC86: 5'-TTTCTTCTGGGTTTGTTTGATTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K102180-0022-748-E05C-MC86
CTCAGCTAATCGAAATCTGAAAATCTGATTATTTTCATTATGTGTTTGATCATATCATAT
CATCATGATCTCAGATTACTTTTGATTGATTCAGTCGCAAGTCTCATGTTTTGATCATAG
CTGAGAACAGGAAAAAGAAGAAAATGGCGTTTGTGACAACTGCGGAAGTTTGTGACGCGA
ATCAAGAGCTGATTCGGAGTGGTCAGCTACGAGCTTTGCAACCCATTTTCCAGATTTATG
GCCGTCGACAAATATTCTCAGGACCAGTTGTCACTGTCAAAGTTTTTGAAGACAATGGCT
TAATCCGTCAATTCATCGAGGAGAAAGGTATTTGCAAATCAAGTTCAAGATTGCAATGGC
TTTGAACAAATTTTACTTGCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCACAATTAT

Last Updated on 06.12.2018 16:11