Sequences from confirmation amplicon


Confirmation sequences for line 748G02 and hit At3g53810 are shown.
Line ID 748G02
Locus At3g53810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19934920
Primers used for PCR DM21 - 8474, show primer details
   
Sequence Name 84-K101748-0022-748-G02C-8409
GenBank Accession LN514407 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K101748-0022-748-G02C-8409
GCCTTTACATTGAAGGTTGTGGAGAAGGATGATACGTTGCCGTTTTGGGAATCTTTGAAC
CGGATTCGTTCAGTACAGAAGGCGTGACCGGTTTTTTGTACCGATGTGTTGGTAAGCTTC
AAGAGACCATTGGGTGTTACTGTTGCGAGACCTTGGAGTGAGATATCTGTTAATGGAGGA
TGGAAACCATTGTAGGTGAAGTTGAGATTTTGAGAGGAAGAAATCATAATTTGACAGAGT
AGGAAGAAGAAGAAGATGAGCTTGAGCTTGACGAACATGGCTGCTTCCGGTTTAATCCGG
TTTAGTTTTACTCTGTTTTGTTAAACGAAAGAGGAAAAGATTGAAGATAGAAATTGAAGT
TATTAAAAGAGGTGAAGAGAAGGATAAGAAAAAAAAGTCGTGGTAGTGTTTCTTTGAGGC
CACGCTTTGTGAGATTTGAATAATATGTCAGTGGTTGGTGAAGTATTAATCAAGATTATA
TAAGCTTGAATTAAATAGAAGTTAATTTCATTCTATGATTAATTGGTTTTATAAGGAGGA
AGTGTATATACAAAAAAAAACCACACTTTGATTTTGACTGATGCATTAATCCAGTTTGTC
TGCATGTGACTTCTAGTGGTC
   
Sequence Name 84-K101754-0022-748-G02C-DM21
GenBank Accession LN514408 [GenBank]
Sequencing primer gene specific primer, DM21: 5'-CTCATTGACCATTGACCACTAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K101754-0022-748-G02C-DM21
ATGCATCAGTCAAATCAAAGTGTGGTTTTTTTTTGTATATACACTTCCTCCTTATAAAAC
CAATTAATCATAGAATGAAATTAACTTCTATTTAATTCAAGCTTATATAATCTTGATTAA
TACTTCACCAACCACTGACATATTATTCAAATCTCACAAAGCGTGGCCTCAAAGAAACAC
TACCACGACTTTTTTTTCTTATCCTTCTCTTCACCTCTTTTAATAACTTCAATTTCTATC
TTCAATCTTTTCCTCTTTCGTTTAACAAAACAGAGTAAAACTAAACCGGATTAAACCGGA
AGCAGCCATGTTCGTCAAGCTCAAGCTCATCTTCTTCTTCTTCCTACTCTGTCAAATTAT
GATTTCTTCCTCTCAAAATCTCAACTTCACCTACAATGGTTTCCATCCTCCATTAACAGA
TATCTCACTCCAAGGTCTCGCAACAGTAACACCCAATGGTCTCTTGAAGCTTACCAACAC
ATCGGTACAAAAAACCGGTCACGCCTTCTGTACTGAACGAATCCGGTTCAAAGATTCCCA
AAACGGCAACGTATCACCCTTCTCCACAACCTTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCANAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11