Sequences from confirmation amplicon


Confirmation sequences for line 749G08 and hit At1g68560 are shown.
Line ID 749G08
Locus At1g68560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25736911
Primers used for PCR CB17 - 8474, show primer details
   
Sequence Name 48-K104623-0022-749-G08C-CB17
GenBank Accession LN506501 [GenBank]
Sequencing primer gene specific primer, CB17: 5'-TAACCACGAAACACTCTTCAACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K104623-0022-749-G08C-CB17
GATCATACCTAGAGATCTCAACTTCATTACAAAAGAAGCTTCACTCTATGGTTTAGGTGA
AAACTCACAAGCTAATGGTATCAAACTCGTACCTAACGAGCCTTATACTCTTTACACTGA
AGATGTTTCAGCTATCAATCTTAACACTGACCTTTATGGTTCGCATCCGATGTATATGGA
TCTAAGAAACGTTGGAGGTAAAGCTTATGCTCATGCTGTTCTTCTTTTGAATAGTAATGG
TATGGATGTTTTCTATAGAGGTGATTCTTTGACGTATAAAGTTATCGGAGGTGTTTTCGA
TTTCTATTTCATCGCCGGACCGTCGCCGTTAAATGTTGTTGATCAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACG
GTTATT
   
Sequence Name 32-K104624-0022-749-G08C-8409
GenBank Accession LN500573 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K104624-0022-749-G08C-8409
GCCTTTACATTGAATATATCCTGATCAACAACATTTAACGGCGACGGTCCGGCGATGAAA
TAGAAATCGAAAACACCTCCGATAACTTTATACGTCAAAGAATCACCTCTATAGAAAACA
TCCATACCATTACTATTCAAAAGAAGAACAGCATGAGCATAAGCTTTACCTCCAACGTTT
CTTAGATCCATATACATCGGATGCGAACCATAAAGGTCAGTGTTAAGATTGATAGCTGAA
ACATCTTCAGTGTAAAGAGTATAAGGCTCGTTAGGTACGAGTTTGATACCATTAGCTTGT
GAGTTTTCACCTAAACCATAGAGTGAAGCTTCTTTTGGTAATGAAGTTGAGATCTCTAGG
TATTGATCTTTGAACACTAATGAGCTTGTTGTGTTGAAGAGTGTTTCGTGGTTA
Primers used for PCR AE09 - 8474, show primer details
   
Sequence Name 78-K118414-0022-749-G08C-AE09
GenBank Accession LN513746 [GenBank]
Sequencing primer gene specific primer, AE09: 5'-CTATAGGGACCAAATAAAATCGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K118414-0022-749-G08C-AE09
TACAAATGGAAGCAAGACAATCTCTCGTTCTTATCTTTAATTGGAAACACCATAATTAGA
AAGCGAAACTTTTCATTATCTTTCCAATATCCCTCAGAAAAGGGTAAAGCTTTTCTATTC
TAAAAGTTGTGTCGGAAAATCAGATACTCTTTACAATGAAATATCAACACTAATGGAACA
TTAATTTAATAGTTTTCGATCTCACTTGGTTTGGTTGATACACAAAATCTATGTTCACGT
GACTCTTCGTTTGACTAAAAGTCAAACTTATAGGAACCTGAACCAATACTAATACTCATC
ATTAAGACATTAACCTATGCTTAAAACACTATAGGACCACAAACATAATCACTAATCCTA
TTTAAATTACCACAAAACATTAAGACAAGAGAGAGAGAAAACAATAACAAAAAAAAACAG
AGCAGTAAATGAACATACCTAAAGACCAGTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
   
Sequence Name 30-K118413-0022-749-G08C-8409
GenBank Accession LN499917 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K118413-0022-749-G08C-8409
TGCTCTGTTTTTTTTTGTTATTGTTTTCTCTCTCTCTTGTCTTAATGTTTTGTGGTAATT
TAAATAGGATTAGTGATTATGTTTGTGGTCCTATAGTGTTTTAAGCATAGGTTAATGTCT
TAATGATGAGTATTAGTATTGGTTCAGGTTCCTATAAGTTTGACTTTTAGTCAAACGAAG
AGTCACGTGAACATAGATTTTGTGTATCAACCAAACCAAGTGAGATCGAAAACTATTAAA
TTAATGTTCCATTAGTGTTGATATTTCATTGTAAAGAGTATCTGATTTTCCGACACAACT
TTTAGAATAGAAAAGCTTTACCCTTTTCTGAGGGATATTGGAAAGATAATGAAAAGTTTC
GCTTTCTAATTATGGTGTTTCCAATTAAAAGATAAGAACGAGAGATTGGTCTTTGCTTTC
CATTTTTGTTGATCTTGATAAGAATGATTATTGCGATTTTATTTGGCCCCTATAG

Last Updated on 06.12.2018 16:11