Genotyping and primer details


Genotyping and primer details for 749G08 - At1g68560
Line ID 749G08
Locus At1g68560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25736911 of line 749G08 Chr1:25736911
   
Insertion allele amplicon 8474 - AE09
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AE09: 5'-CTATAGGGACCAAATAAAATCGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25736437
   
Insertion allele amplicon 8474 - CB17
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CB17: 5'-TAACCACGAAACACTCTTCAACAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25737368
   
wt amplicon (over InsPos) AE09 - CB17
wt amplicon start Chr1:25736437
wt amplicon end Chr1:25737368
wt amplicon size 932 bp
Locus specific primer CB17: 5'-TAACCACGAAACACTCTTCAACAC-3'
2nd locus specific primer AE09: 5'-CTATAGGGACCAAATAAAATCGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11