Sequences from confirmation amplicon


Confirmation sequences for line 750A03 and hit At3g14050 are shown.
Line ID 750A03
Locus At3g14050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4650921
Primers used for PCR DN43 - 8474, show primer details
   
Sequence Name 40-K101830-0022-750-A03C-8409
GenBank Accession LN503659 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K101830-0022-750-A03C-8409
GTTTCTTTTTCTTTTTCTTTTGGTCGGATGGGGAAGCTGATGGGTCCTCAAATTGTTAAC
TAGTCCATGTATCATGTATGTATGTATATATATAGTCCCCATATGATTAATTTGTTGTAC
ATAACAATGGCTCCGTTGGTTCAATGTAAGCTTAATAAAAAGAAAGCAAAAAGTTAGTAT
TAGACTATATAAATTTGTCAAAAAAAATAAACGTTATTTCATTAAGAGTTCTAAAATTTT
ATTGAGACACTGACACAGTGTGAAAATGTTTTGAAGTATCAAACCTTGTCTTGATGAGGC
CGGTTCTTTTTCGCCTTCTATTTACCCAAAACTATTGAGACTTAAACTTCAAACTAGTAG
AATAAAGAGGCAGATTTCTCTCATCAATGTATTAATTTGTAGTTGAATTATTCACCATAC
CTTATCTTTAATATATGATTTTGGTCAAAAATCTATAAATATATCTTGAGTGATGCCAAA
AAAAAGGGATCGGCCTCCATGAATTGGTCATTCTTCTGCCTTGAGCACAATCCTCGGAAT
TATTTTTAGCTAATGCAAGAGTTTGTGGTTTTCTATCTCCCTTTGCTCCTCTATAAAACG
GACTTTCGAATCATCA
   
Sequence Name 84-K101833-0022-750-A03C-DN43
GenBank Accession LN514409 [GenBank]
Sequencing primer gene specific primer, DN43: 5'-TGTTTTGTTCTGATGATTCGAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K101833-0022-750-A03C-DN43
AAACTCTTGCATTAGCTAAAAATAATTCCGAGGATTGTGCTCAAGGCAGAAGAATGACCA
ATTCATGGAGGCCGATCCCTTTTTTTTGGCATCACTCAAGATATATTTATAGATTTTTGA
CCAAAATCATATATTAAAGATAAGGTATGGTGAATAATTCAACTACAAATTAATACATTG
ATGAGAGAAATCTGCCTCTTTATTCTACTAGTTTGAAGTTTAAGTCTCAATAGTTTTGGG
TAAATAGAAGGCGAAAAAGAACCGGCCTCATCAAGACAAGGTTTGATACTTCAAAACATT
TTCACACTGTGTCAGTGTCTCAATAAAATTTTAGAACTCTTAATGAAATAACGTTTATTT
TTTTTGACAAATTTATATAGTCTAATACTAACTTTTTGCTTTCTTTTTATTAAGCTTACA
TTGAACCAACGGAGCCATTGTTATGTACAACAAATTAATCATATGGGGACTATATATATA
CATACATACATGATACATGGACTAGTTAACAATTTGAGGACCCATCAGCTTCCCCATCCG
ACCAAAAGAAAAAGAAAAAGAAACATGTATAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11