Genotyping and primer details


Genotyping and primer details for 750A03 - At3g14050
Line ID 750A03
Locus At3g14050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4650921 of line 750A03 Chr3:4650921
   
Insertion allele amplicon 8474 - DN43
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DN43: 5'-TGTTTTGTTCTGATGATTCGAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4650316
   
wt amplicon (over InsPos) 70dy - DN43
wt amplicon start Chr3:4650316
wt amplicon end Chr3:4651336
wt amplicon size 1021 bp
Locus specific primer DN43: 5'-TGTTTTGTTCTGATGATTCGAAAG-3'
2nd locus specific primer 70dy: 5'-TACAAGCCTAACAGTAGCCAGGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11