Sequences from confirmation amplicon


Confirmation sequences for line 752H11 and hit At1g32510 are shown.
Line ID 752H11
Locus At1g32510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11757247
Primers used for PCR CR85 - 8474, show primer details
   
Sequence Name 53-K107248-0022-752-H11C-CR85
GenBank Accession LN508173 [GenBank]
Sequencing primer gene specific primer, CR85: 5'-AGACAAACGTGTGTTACTTGCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K107248-0022-752-H11C-CR85
CTTGACAAATTGTTACATTATTGCTTAATGACTCATCTGAAACCTCTGGTGAGATAATTT
CACATTTCTTAAGCGTATGCTCATTCCTCTTTATCACACGACACAGCGCGAAGTCTCCCT
AAAGAGCGGAAGAATACCAAATGAAAATGATCAATTGGTAAATGGTAAACTATGAATCAA
GTTTTTTATTTTTTCATCTGTTTGGTTTATGATGTTGTTACCTTGAAGTTTAGTGATCCT
TGTGAAGTATCGTTGTCGAAGAGGCGATACTCATGCATTACCCACTCGGTTCTACCACCA
AAAGGAGCTCGACCCATATAAAAAACTAGGGTTTTTCGGCAGCCGGTGATAGAGGAAGAT
GATGAAGAAGAAGAAGAGTGGCAAACGATTTTTCGATCTTTGCCAGTGGCTTTCCAATAA
CCAGATTTAGTTGCTCTGTTTATTCGAGATCCGTTCTGGTATTTTCTGTCGCGATGACAG
AAGAAAAACCATTCCATATCTCGATTTGAATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 37-K107247-0022-752-H11C-8409
GenBank Accession LN502636 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K107247-0022-752-H11C-8409
GAGCCATTTACAATTGAATATATTCAAATCGAGATATGGAATGGTTTTTCTTCTGTCATC
GCGACAGAAAATACCAGAACGGATCTCGAATAAACAGAGCAACTAAATCTGGTTATTGGA
AAGCCACTGGCAAAGATCGAAAAATCGTTTGCCACTCTTCTTCTTCTTCATCATCTTCCT
CTATCACCGGCTGCCGAAAAACCCTAGTTTTTTATATGGGTCGAGCTCCTTTTGGTGGTA
GAACCGAGTGGGTAATGCATGAGTATCGCCTCTTCGACAACGATACTTCACAAGGATCAC
TAAACTTCAAGGTAACAACATCATAAACCAAACAGATGAAAAAATAAAAAACTTGATTCA
TAGTTTACCATTTACCAATTGATCATTTTCATTTGGTATTCTTCCGCTCTTTAGGGAGAC
TTCGCGCTGTGTCGTGTGATAAAGAGGAATGAGCATACGCTTAAGAAATGTGAAATTATC
TCACCAGAGGTTTCAGATGAGTCATTAAGCAATAATGTTAACAATTTTTGTCAAGCAAGT
GACCTAGAAAAGGGTTCTTGTGATGCAAGTAACACGNNTTTGTC

Last Updated on 06.12.2018 16:11