Sequences from confirmation amplicon


Confirmation sequences for line 755E11 and hit At3g61400 are shown.
Line ID 755E11
Locus At3g61400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22719801
Primers used for PCR CR87 - 8474, show primer details
   
Sequence Name 56-K107248-0022-755-E11C-CR87
GenBank Accession LN509119 [GenBank]
Sequencing primer gene specific primer, CR87: 5'-TAAACGGAGTAACGCAGAGGTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K107248-0022-755-E11C-CR87
GATGGTGGTGTCCCTATACTTGGGAGGGTTTTGTTCTGACAGAAGCTCTTTTATGGGTCC
ATATACTCGATGGCTCTCCCTCATCACGGTGCTGAAGAAACATGGCACTGAAATCCGCGG
TTCAGCCGCTCTATTTGCTATCACCCTGTGCTCCACGCTTATGAACTTGCCATTGCTTAT
AAGCTGCACAAATTCAAATAAAAAATCAACTCACATCTCCATTCAAATCATAACTAGCCT
ATATCTTTTACTTACTACTTTAAATTCCATATTATTTCATATATGATATTCCTAACAAAG
ATAGAGCTAAAATGTTACAAAGCTAAAAAGTCACTTCTTTAACCTGGAGAAGATCTCCGA
GGTTAACGACAAGAGCCCCAGGGACGGGAGGAATATCGATCCAATATTGTTTATCATGGA
GAACTTGAAGCCCACCGAGATTCCCCTGAAGAACAATGGTGAGAAAAGAAAAATCGGTGT
GCTTGCTTAAGCCTAAAGTATGGTCAGGCTGCGGACAAGGTGGATAGTCTACATGTATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATT
   
Sequence Name 40-K107247-0022-755-E11C-8409
GenBank Accession LN503727 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K107247-0022-755-E11C-8409
GCCATTTACAATTGAATACATGTAGACTATCCACCTTGTCCGCAGCCTGACCATACTTTA
GGCTTAAGCAAGCACACCGATTTTTCTTTTCTCACCATTGTTCTTCAGGGGAATCTCGGT
GGGCTTCAAGTTCTCCATGATAAACAATATTGGATCGATATTCCTCCCGTCCCTGGGGCT
CTTGTCGTTAACCTCGGAGATCTTCTCCAGGTTAAAGAAGTGACTTTTTAGCTTTGTAAC
ATTTTAGCTCTATCTTTGTTAGGAATATCATATATGAAATAATATGGAATTTAAAGTAGT
AAGTAAAAGATATAGGCTAGTTATGATTTGAATGGAGATGTGAGTTGATTTTTTATTTGA
ATTTGTGCAGCTTATAAGCAATGGCAAGTTCATAAGCGTGGAGCACAGGGTGATAGCAAA
TAGAGCGGCTGAACCGCGGATTTCAGTGCCATGTTTCTTCAGCACCGTGATGAGGGAGAG
CCATCGAGTATATGGACCCATAAAAGAGCTTCTGTCAGAACAAAACCCTCCCAAGTATAG
GGACACCACCATCTCCGAGTTCGCAAGCATGTACGCGTCTAAAGAGATCAATACCTCTGC
GTTACTCCGTTT

Last Updated on 06.12.2018 16:11