Sequences from confirmation amplicon


Confirmation sequences for line 757A06 and hit At5g16480 are shown.
Line ID 757A06
Locus At5g16480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5382208
Primers used for PCR NL72 - 8474, show primer details
   
Sequence Name 67-K103789-0022-757-A06C-NL72
GenBank Accession LN511800 [GenBank]
Sequencing primer gene specific primer, NL72: 5'-TGAGACAGCTAAAAGAGAAAAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K103789-0022-757-A06C-NL72
GTTCTTACCACTAGTACTCTAGAGCACTCAACACTGTATCCTTTGGCATAGGAGTTGGAG
GATCCTATTGGCGTAAAAGTAAAACAATCCAAACTCAGAAATCAAAGAAGCTATAACAGG
AACAGAACAGGGTATTTATATGTTTCTTAAAGGTCTTATAAGATTCATCAACTCAAATGC
TACAAGCCAACAAGACACTAAAAACAGGGTCAAATGTTCTTGAAGAAGAACTCAAAAAGA
TTCAATACAATTGCTGTTGTTGAATGAATCATAAAGGTTAGAAAATCATACCGTTTTGCC
TTCAATCCCGAACTGGAAAAGCTTAATGTTGCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCANAAATGTA
   
Sequence Name 75-K103790-0022-757-A06C-8409
GenBank Accession LN513230 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K103790-0022-757-A06C-8409
GCCTTTACATTGAATATATCCTGCACATTAAGCTTTTCCAGTTCGGGATTGAAGGCAAAA
CGGTATGATTTTCTAACCTTTATGATTCATTCAACAACAGCAATTGTATTGAATCTTTTT
GAGTTCTTCTTCAAGAACATTTGACCCTGTTTTTAGTGTCTTGTTGGCTTGTAGCATTTG
AGTTGATGAATCTTATAAGACCTTTAAGAAACATATAAATACCCTGTTCTGTTCCTGTTA
TAGCTTCTTTGATTTCTGAGTTTGGATTGTTTTACTTTTACGCCAATAGGATCCTCCAAC
TCCTATGCCAAAGGATACAGTGTTGAGTGCTCTTAGAGTACTAGTTGGTAAGGAACCAGA
AGAGACTCTTTGATGTGTTGTCGGCTTTTCTCTTTTAGCTGTCTC
Primers used for PCR 26au - 8474, show primer details
   
Sequence Name 68-K118446-0022-757-A06C-26au
GenBank Accession LN512098 [GenBank]
Sequencing primer gene specific primer, 26au: 5'-GGATGATGATAACGATGGAGAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K118446-0022-757-A06C-26au
TAGAGATGAATTTACCGATCTGGGTTTCCTGAGCTGGAGAATTTCGGATTCTTATCAACC
CTAAATCTTCGATCCATCATGTAAGATTCTCTTCTTTCATTCTTCTGAATCTTTTGTTAA
AAATTAAAAAAAGAAGAAGAATCTTTTATTTAATAACGCATCGTTGTCGTTGTTTATAGA
TAGACAGATTCCTTAAATCACCACTTTCACTTTCATGGCTTTTATTAGTTAAATGTCAAA
AAAATGTGGGATAATTATATATTTGTGTTGTTGAAATTTGACCCACGCAGAAAGATTTGA
TTACAAAAACGAGTTTTGTTTGTTGGGTTTGTCTGGAAACAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTA
TT
   
Sequence Name 28-K118445-0022-757-A06C-8409
GenBank Accession LN499048 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118445-0022-757-A06C-8409
CATTTACATTGAATATATCCTGTTTCCAGACAAACCCAACAAACAAAACTCNGTTTTTGT
AATCAAATCTTTCTGCGTGGGTCAAATTTCAACAACACAAATATATAATTATCCCACATT
TTTTTGACATTTAACTAATAAAAGCCATGAAAGTGAAAGTGGTGATTTAAGGAATCTGTC
TATCTATAAACAACGACAACGATGCGTTATTAAATAAAAGATTCTTCTTCTTTTTTTAAT
TTTTAACAAAAGATTCAGAAGAATGAAAGAAGAGAATCTTACATGATGGATCGAAGATTT
AGGGTTGATAAGAATCCGAAATTCTCCAGCTCAGGAAACCCAGATCGGTAAATTTCATCT
TCTACCATTGAAAAATTCGGCGGCGGAATCAAAACTTCTCCATCGTATTCATCATC

Last Updated on 06.12.2018 16:11