Sequences from confirmation amplicon


Confirmation sequences for line 757F04 and hit At5g22950 are shown.
Line ID 757F04
Locus At5g22950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7681378
Primers used for PCR 19lq - 8474, show primer details
   
Sequence Name 38-K114022-0022-757-F04C-19lq
GenBank Accession LN503060 [GenBank]
Sequencing primer gene specific primer, 19lq: 5'-GTTCTTCTCGAACTCACAATTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K114022-0022-757-F04C-19lq
GAGAGCAAACTTTCTCTTTGCAGCTTACAAGACAGAGACTTTTTGGGTTCTAACCTTAGC
AGAGACCATATCATTCCTCTTTGCAGCTTCTTTAATAGCTTTCTGTACATTTCTCTCTTC
TTTCTGTATATCTGAACAAAATCAAAACTTTAAAAATTCCATAGATTCAAACCCAACCCA
TATGATCAAATCGAAAAATTCATACACCAATCAAAATCATCCGATCCAAATCTGATTCAG
AGTAAATTCTCGCAATTTTTGGGTGCGATCAACCAGATTCAATCCAATTAATCTAAACTA
GCATCTAATTCAATTAGATTTCGAAAACCCTAAATTGACCATAAGAAAAAGGCGAGAGAA
GATGTGTAAAATAGAAGAACCTCGGATTTGGCGTTCGATGTTACGACACTCCTGACGTAA
TTTGCGTTGCCAATCACGCAACAGTTGTTTGGGATCAGGTTTTGGCTTTATGATGTTCAT
CACTCTCTCCATTGGATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 17-K114021-0022-757-F04C-8409
GenBank Accession LN493936 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114021-0022-757-F04C-8409
TCCTGATCCAATGGAGAGAGTGATGAACATCATAAAGCCAAAACCTGATCCCAAACAACT
GTTGCGTGATTGGCAACGCAAATTACGTCAGGAGTGTCGTAACATCGAACGCCAAATCCG
AGGTTCTTCTATTTTACACATCTTCTCTCGCCTTTTTCTTATGGTCAATTTAGGGTTTTC
GAAATCTAATTGAATTAGATGCTAGTTTAGATTAATTGGATTGAATCTGGTTGATCGCAC
CCAAAAATTGCGAGAATTTACTCTGAATCAGATTTGGATCGGATGATTTTGATTGGTGTA
TGAATTTTTCGATTTGATCATATGGGTTGGGTTTGAATCTATGGAATTTTTAAAGTTTTG
ATTTTGTTCAGATATACAGAAAGAAGAGAGAAATGTACAGAAAGCTATTAAAGAAGCTGC
AAAGAGGAATGATATGGTCTCTGCTAAGGTTAGAACCCAAAAAGTCTCTGTCTTTGTAAG
CTGCAAAGAGAAAGTTTTGCTCTCTCTTCTGATTTGAGTCTTTGGTGTTCTTAGGCACTT
GCTAAGGAAATTGTGAGTTCNAGAAGAACACNAAAGACTCAAATCAGGAGAGAGAG

Last Updated on 06.12.2018 16:11