Genotyping and primer details


Genotyping and primer details for 757F04 - At5g22950
Line ID 757F04
Locus At5g22950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7681378 of line 757F04 Chr5:7681378
   
Insertion allele amplicon 8474 - 19lq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19lq: 5'-GTTCTTCTCGAACTCACAATTTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7681938
   
wt amplicon (over InsPos) 08mj - 19lq
wt amplicon start Chr5:7680879
wt amplicon end Chr5:7681938
wt amplicon size 1060 bp
Locus specific primer 19lq: 5'-GTTCTTCTCGAACTCACAATTTCC-3'
2nd locus specific primer 08mj: 5'-CAGTTGTTTTGTTCTAAAATCGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11