Sequences from confirmation amplicon


Confirmation sequences for line 759F12 and hit At5g37660 are shown.
Line ID 759F12
Locus At5g37660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14960182
Primers used for PCR CR91 - 8474, show primer details
   
Sequence Name 57-K107248-0022-759-F12C-CR91
GenBank Accession LN509435 [GenBank]
Sequencing primer gene specific primer, CR91: 5'-TGCACCATCAGTTGAATATCTAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K107248-0022-759-F12C-CR91
GAGAGGCCGTGCCACAATCATTCTTAAGCCGTCCATGGCCGTTCCTAAACAATCTTGGCA
CTCTGACACCGTTAGATCTCCTACACATTGTCCCATACCTTGAACATCACCGGACCCTCC
GGCTCGAAAATACCCTCCGCCATTGACTAGCTCCGTGAGAACTGCGTCTCTCCGGCTGAT
ACCGTCGGTATTATAACCCTCAGAGGATCCACACTTCTTTAGGACAACGGTCTTGTCTTC
TTGGCCAAGGAAGCTGATGTTATCGTATTTGATGTAGCAGCCAGCTAGCTGGAGGGCTCC
ACCACAAGTGAAAGGGCAAAGAGGACAATTGGACATGTTATCATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATT
   
Sequence Name 41-K107247-0022-759-F12C-8409
GenBank Accession LN504097 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K107247-0022-759-F12C-8409
GCCTTTACAATTGAATATGATAACATGTCCAATTGTCCTCTTTGCCCTTTCACTTGTGGT
GGAGCCCTCCAGCTAGCTGGCTGCTACATCAAATACGATAACATCAGCTTCCTTGGCCAA
GAAGACAAGACCGTTGTCCTAAAGAAGTGTGGATCCTCTGAGGGTTATAATACCGACGGT
ATCAGCCGGAGAGACGCAGTTCTCACGGAGCTAGTCAATGGCGGAGGGTATTTTCGAGCC
GGAGGGTCCGGTGATGTTCAAGGTATGGGACAATGTGTAGGAGATCTAACGGTGTCAGAG
TGCCAAGATTGTTTAGGAACGGCCATTGGACGGCTTAAGAATGATTGTGGCACGGCCGTC
TTCGGAGACATGTTCTTGGCTAAGTGTTACGCTAGATATTCAACTGATGGTGCA

Last Updated on 06.12.2018 16:11