Sequences from confirmation amplicon


Confirmation sequences for line 760A07 and hit At5g44320 are shown.
Line ID 760A07
Locus At5g44320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17855344
Primers used for PCR 71ra - 8474, show primer details
   
Sequence Name 39-K115781-0022-760-A07C-71ra
GenBank Accession LN503473 [GenBank]
Sequencing primer gene specific primer, 71ra: 5'-TGAACCAACATTTTGGAACATAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K115781-0022-760-A07C-71ra
CAGCTTCGACATTTTTTAAAGTAACTCAAAACAACACGACTTGAAATATACCCATATCAT
CAAAATCAGGCTTCAACTTCGGATTTCTTATCCTCTACAGCTACATTAGTAGAGGAAACC
CCATTAGTCTCTGCTCCTGCATCAGTGTTCTCCTCAGGTGGCTGAACCTGTTCATCTTCA
GGGAGTGGCTCCTCCACATAATCATTGTCAAATGCATCAGCCGGAACTTCGTAAATCCTA
ACTTGAGGCTTCGATGGATCCTTCACTAGAACATACTTTCCTTCACTTAACTTCATACAC
AGATCAACAATCGACTTCACAATTCCCCACATATTGTTAGTGTTCAGATTGATCTGCCCA
GCGAAATCTTTCGGCTTATACCCCAAAACCGATNATATAACATGGTTAAAATGATCACGA
GGATGAACCCTCGAAACAAACCCAATCTTCATCATATCCGCATTAGCCAAGAGAGCCTGC
GCAGTCCATTTAGCTAACTTATTACCATTTAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 15-K115780-0022-760-A07C-8409
GenBank Accession LN493116 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K115780-0022-760-A07C-8409
CCTAAATGGTAATAAGTTAGCTAAATGGACTGCGCAGGCTCTCTTGGCTAATGCGGATAT
GATGAAGATTGGGTTTGTTTCGAGGGTTCATCCTCGTGATCATTTTAACCATGTTATATT
ATCGGTTTTGGGGTATAAGCCGAAAGATTTCGCTGGGCAGATCAATCTGAACACTAACAA
TATGTGGGGAATTGTGAAGTCGATTGTTGATCTGTGTATGAAGTTAAGTGAAGGAAAGTA
TGTTCTAGTGAAGGATCCATCGAAGCCTCAAGTTAGGATTTACGAAGTTCCGGCTGATGC
ATTTGACAATGATTATGTGGAGGAGCCACTCCCTGAAGATGAACAGGTTCAGCCACCTGA
GGAGAACACTGATGCAGGAGCAGAGACTAATGGGGTTTCCTCTACTAATGTAGCTGTAGA
GGATAAGAAATCCGAAGTTGAAGCCTGATTTTGATGATATGGGTATATTTCAAGTCGTGT
TGTTTTGAGTTACTTTTAAAAAATGTTCGAAAGCTTGTTCACTTTTTCATAGACTATTTA
TGTTC

Last Updated on 06.12.2018 16:11