Sequences from confirmation amplicon


Confirmation sequences for line 760E09 and hit At3g55240 are shown.
Line ID 760E09
Locus At3g55240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20474006
Primers used for PCR NB20 - 8474, show primer details
   
Sequence Name 86-K101222-0022-760-E09C-8409
GenBank Accession LN514628 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K101222-0022-760-E09C-8409
TCGGACTGAGCCATTTCATGTAGAAAGAGAACAGGAATTCTTCAAGGCGTATGAGGAGAG
GCAAAGCAAACAAGAGCAAATGTCGGAGGAAGAGACAAACCAGATGATCCAGAAGATTAT
CTCGGATTCATCTAAAGAATCCGACGACTGATCGCGATGTTCGATCGTTAGATTTCGAAA
ACCTTATAGTTTATATATATGAATCTCATGAAAACGATTTATCCCTGACGCATGCATGTT
TATAAGTAAATCGTTTTTTCATCTTAGCATCGGTCCAACAAGCAAGACAGCAAAAAAAGG
AGTGATGACACTCTTTTTGGTACGTTATACGTACGTGGTCCACATAATATTGCCTCTGTT
TAATTATATATAATGAAACGGACGCTAGCTCATACATGGATCGATGGTGTGGTGTTTANT
TTGAATG
   
Sequence Name 86-K101229-0022-760-E09C-NB20
GenBank Accession LN514629 [GenBank]
Sequencing primer gene specific primer, NB20: 5'-ACATTCAATTAAAACACCACACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K101229-0022-760-E09C-NB20
GTCCGTTTATTATATATATTAAACAGAGGCAATATTATGTGGACCACGTACGTATAACGT
ACCAAAAAGAGTGTCATCACTCCTTTTTTTGCTGTCTTGCTTGTTGGACCGATGCTAAGA
TGAAAAAACGATTTACTTATAAACATGCATGCGTCAGGGATAAATCGTTTTCATGAGATT
CATATATATAAACTATAAGGTTTTCGAAATCTAACGATCGAACATCGCGATCAGTCGTCG
GATTCTTTAGATGAATCCGAGATAATCTTCTGGATCATCTGGTTTGTCTCTTCCTCCGAC
ATTTGCTCTTGTTTGCTTTGCCTCTCCTCATACGCCTTGAAGAATTCCTTGTTCTCTTTC
TACATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAG

Last Updated on 06.12.2018 16:11