Sequences from confirmation amplicon


Confirmation sequences for line 763E12 and hit At2g30010 are shown.
Line ID 763E12
Locus At2g30010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12805890
Primers used for PCR 72ra - 8474, show primer details
   
Sequence Name 47-K115914-0022-763-E12C-72ra
GenBank Accession LN506300 [GenBank]
Sequencing primer gene specific primer, 72ra: 5'-AAAATTCATTTGCCAAAGCATAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K115914-0022-763-E12C-72ra
TCAATAAAATGAAATTTTCTTTCCTCAAAAACAAGACAACTAAAATTAACATTAACCAAA
TTAATCTAACAAAGTAGAGCTAGTGACAAGTGAGTGAGTTTAAATAACCTAGGGACATTG
CAGTTGAAAGGTTTCCACCGGAACTTGAGATAGTCGGAGTCAGGTCTGCCGTAAGTCTGG
CAGTCGAATCCCGGATCTATGATTCCTCGGCCGCATTCTTTAGACCGGTAAAGTGGGTAA
GTCTCATCCCGCACCCACTCGCCGGCGAACAGATCACAACTACTATGATTCACCGGGAAA
ACCACCTGACTTCGGCGAGGATGCTTCACGAAGTTGCTGTGGTTGCTTTGGATCGGCCGC
CGGAAGAGAGGGAGAGGTGAAGCTGAGAAATCTACATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 21-K115913-0022-763-E12C-8409
GenBank Accession LN495803 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K115913-0022-763-E12C-8409
AGATTTCTCAGCTTCACCTCTCCCTCTCTTCCGGCGGCCGATCCAAAGCAACCACAGCAA
CTTCGTGAAGCATCCTCGCCGAAGTCAGGTGGTTTTCCCGGTGAATCATAGTAGTTGTGA
TCTGTTCGCCGGCGAGTGGGTGCGGGATGAGACTTACCCACTTTACCGGTCTAAAGAATG
CGGCCGAGGAATCATAGATCCGGGATTCGACTGCCAGACTTACGGCAGACCTGACTCCGA
CTATCTCAAGTTCCGGTGGAAACCTTTCAACTGCAATGTCCCTAGGTTATTTAAACTCAC
TCACTTGTCACTAGCTCTACTTTGTTAGATTAATTTGGTTAATGTTAATTTTAGTTGTCT
TGTTTTTGAGGAAGAAATATTCATTTTATTGATACCGAGGTTATAATCTGACATCCAATA
GTAAAACTTATGCTTT

Last Updated on 06.12.2018 16:11