Genotyping and primer details


Genotyping and primer details for 763E12 - At2g30010
Line ID 763E12
Locus At2g30010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12805890 of line 763E12 Chr2:12805890
   
Insertion allele amplicon 8474 - 72ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 72ra: 5'-AAAATTCATTTGCCAAAGCATAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12806332
   
wt amplicon (over InsPos) 24rt - 72ra
wt amplicon start Chr2:12805531
wt amplicon end Chr2:12806332
wt amplicon size 802 bp
Locus specific primer 72ra: 5'-AAAATTCATTTGCCAAAGCATAAG-3'
2nd locus specific primer 24rt: 5'-TTTATATGTGTTTCCCAAAACCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11