Sequences from confirmation amplicon


Confirmation sequences for line 764B10 and hit At4g28220 are shown.
Line ID 764B10
Locus At4g28220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13994931
Primers used for PCR 02ck - 8474, show primer details
   
Sequence Name 17-K108223-0022-764-B10C-02ck
GenBank Accession LN493882 [GenBank]
Sequencing primer gene specific primer, 02ck: 5'-GAACGTATCAGTTCATTTGCTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K108223-0022-764-B10C-02ck
TTGATGTCGACTGGATGCGTGTATGTCTGTGACTGATAAAGATATCACAGTGAAAGTCAA
ATCAAGTGGAGAACTCGTAAGTATTCCTCATGGATTGATATTGTGGTCCACTGGAGTTGG
AACCCGTCCAGTTATCAGCGACTTCATGGAGCAAGTTGGCCAAGGAGGCAGACGTGCGGT
GGCAACTAACGAGTGGTTACAAGTGACAGGATGTGAGAATGTATATGCAGTTGGCGATTG
TGCTTCCATAGCTCAACGAAAAATCTTGGTCTGTGAACCCCATTAGACTGTTGATGCTAT
TCCCTTCTTTTTTTCATATGCATGCTAAATCTTGATAGATTCTTGTGAATCTTAATTTAG
GGGGATATTGCAAACATATTCAAAGCTGCAGATGCGGACAACTCAGGAACCTTGACCATG
GAAGAGTTAGAAGGCGTGGTTGATGATATTATTGTGAGGTATCCGCAGGTGGAGCTGCAA
TGAAAGATTCAATTGTAAATGACTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTA
   
Sequence Name 01-K108222-0022-764-B10C-8409
GenBank Accession LN486605 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K108222-0022-764-B10C-8409
TTTACAATTGAATCTTTCATTGCAGCTCCACCTGCGGATACCTCACAATAATATCATCAA
CCACGCCTTCTAACTCTTCCATGGTCAAGGTTCCTGAGTTGTCCGCATCTGCAGCTTTGA
ATATGTTTGCAATATCCCCCTAAATTAAGATTCACAAGAATCTATCAAGATTTAGCATGC
ATATGAAAAAAAGAAGGGAATAGCATCAACAGTCTAATGGGGTTCACAGACCAAGATTTT
TCGTTGAGCTATGGAAGCACAATCGCCAACTGCATATACATTCTCACATCCTGTCACTTG
TAACCACTCGTTAGTTGCCACCGCACGTCTGCCTCCTTGGCCAACTTGCTCCATGAAGTC
GCTGATAACTGGACGGGTTCCAACTCCAGTGGACCACAATATCAATCCATGAGGAATACT
TACGAGTTCTCCACTTGATTTGACTTTCACTGTGATATCTTTATCAGTCACAGACATTAC
ACGCATTCCAGTCTGAACATCAATACCATCTCGCGTGAACTTTTGTTCAGCAAATGAACT
GATACGTT

Last Updated on 06.12.2018 16:11