Sequences from confirmation amplicon


Confirmation sequences for line 764C01 and hit At3g17970 are shown.
Line ID 764C01
Locus At3g17970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6150754
Primers used for PCR AC09 - 8474, show primer details
   
Sequence Name 43-K027791-022-764-C01C-8409
GenBank Accession LN504718 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K027791-022-764-C01C-8409
CTTTACATTTTTAATCTCTCTCTTTCTCTCTCTCACCTGCCATGCTAGTAAAGTTCCCCT
CACACATGTTAAAAGCAACCACTGACCAGTCAGTGTTAGCAAAACCAATCCACAGTATAT
GTGTCTTACATTTATCGTGCTGATGATTCAGGTGACTGTGCCACTCGGACATCATGAGAA
ATGCCCTATTTCAGTTTCTTTCATAGGAAGACACGGTGGTGACCGCTTTTTACTAGATAC
AGTGCAGACAATGTATCCATCTTTGCAAGAGTACTCGAGTATTGTTACCGATCCCAAATC
TTCTAAAAAGGCTATCACCAAAGAAGAGTCAGCCGAGATTGCCAAAGAGAAGGTCAATTA
ATATCCATTGCATGTCTCATTTTCTTAATGGCATTATCATCCATAATCTAATGTGGTTAT
TATATCTTTGTGTCCCGTGATTATGTAAACCAGGGTAACCAAGCGTTCAAAGAGAAACTA
T
   
Sequence Name 44-K027791-022-764-C01C-AC09
GenBank Accession LN505061 [GenBank]
Sequencing primer gene specific primer, AC09: 5'-CCAATAGCTTTCTGCCATAGTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K027791-022-764-C01C-AC09
ATAATCACGGGACCAAAGATATAATAACCACATTAGATTATGGATGATAATGCCATTAAG
AAAATGAGACATGCAATGGATATTAATTGACCTTCTCTTTGGCAATCTCGGCTGACTCTT
CTTTGGTGATAGCCTTTTTAGAAGATTTGGGATCGGTAACAATACTCGAGTACTCTTGCA
AAGATGGATACATTGTCTGCACTGTATCTAGTAAAAAGCGGTCACCACCGTGTCTTCCTA
TGAAAGAAACTGAAATAGGGCATTTCTCATGATGTCCGAGTGGCACAGTCACCTGAATCA
TCAGCACGATAAATGTAAGACACATATACTGTGGATTGGTTTTGCTAACACTGACTGGTC
AGTGGTTGCTTTTAACATGTGTGAGGGGAACTTTACTAGCATGGCAGGTGAGAGAGAGAA
AGAGAGAGATTTAAAAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTA
Primers used for PCR AE06 - 8474, show primer details
   
Sequence Name 50-K118366-0022-764-C01C-AE06
GenBank Accession LN507315 [GenBank]
Sequencing primer gene specific primer, AE06: 5'-GGTGTGCCTATTGTTTATTCACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K118366-0022-764-C01C-AE06
CCTCTATAACATATTGGCATTGTAGGCACGAGTTCCTTCAAATCATGGGGATTGGATCAA
CACAGTGAAGCCAGCCATTGATCCTGTGATTTTATCACAAGTGTGTGAGAACCCAGAACT
AACCAATGAAGAGACTGAGAACCTGAATGCTATCAGAAACGAGACTCGAGTGGCTATTGG
TTCACTTCTCAAGGTATTCCTGCTCTTCCATGACATGTCCACAACTTGAGCGAGTTAGTT
TATATGTCTCTTGACTAATCTCTAATGGTTCCCAACTCTGTAACGTTATGTATCAAGGAT
GATGGCATTCTGGTAATTCCAACATTGCCAGCTGTTCCTCCAAAACTTGGTAGCAAGGAG
ATAACATCTGAAGACTATCAAAACCGAGCTTCAAGTCTGCTTAGCATTGCTAGCATATCA
GGATGTTGTTAGCCATTTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 71-K118365-0022-764-C01C-8409
GenBank Accession LN512630 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K118365-0022-764-C01C-8409
TTGAATATATCCTGTAAATGGCTAACAACATCCTGATATGCTAGCAATGCTAAGCAGACT
TGAAGCTCGGTTTTGATAGTCTTCAGATGTTATCTCCTTGCTACCAAGTTTTGGAGGAAC
AGCTGGCAATGTTGGAATTACCAGAATGCCATCATCCTTGATACATAACGTTACAGAGTT
GGGAACCATTAGAGATTAGTCAAGAGACATATAAACTAACTCGCTCAAGTTGTGGACATG
TCATGGAAGAGCAGGAATACCTTGAGAAGTGAACCAATAGCCACTCGAGTCTCGTTTCTG
ATAGCATTCAGGTTCTCAGTCTCTTCATTGGTTAGTTCTGGGTTCTCACACACTTGTGAT
AAAATCACAGGATCAATGGCTGGCTTCACTGTGTTGATCCAATCCCCATGATTTTGAAGG
AACTCGTGCCTACAATGCCAATATTGTTATAGAGTGTTCAAAACTAAGATATTTTCATCC
TCTGTAGGTGAATAAACATAGGGCACAC

Last Updated on 06.12.2018 16:11