Sequences from confirmation amplicon


Confirmation sequences for line 764D11 and hit At1g35520 are shown.
Line ID 764D11
Locus At1g35520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:13084211
Primers used for PCR KQ05 - 8474, show primer details
   
Sequence Name 57-K034240-022-764-D11C-8409
GenBank Accession LN509339 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K034240-022-764-D11C-8409
ATTTCCCGGGACTGAAGCCATTTACAATTGGGTAATTACTCNTTTCTTTTTCTCCATATT
TACATTTTGTTCAATTGATTAAGATATTTTGGGACAATTATTGGAGTTAGTAATTTCTCT
CCTCATTGGAAGTGTTCAGACTGGCGCAGCTTAGAAGTAAATTTTCATTCACACTTTATA
ACATTCTTTTAGGAATACGTTTTTGAGTGCAATAAATAACATATCACTAATTAACTTTAA
TTTTGCAGGTGCAGTGGGATGAATTTGCATCATTTTTGAGACCTAATAAAGTGTCACCCT
GGGAGATCGAACATTTAATGCCTGCGTTAAATGTTCCGCGATCATCTTTTCTCAAGAACA
AGCGTTTACGAGAAGTTAATGAATTCGGTATAAAAATACACTCTTATAATCTTTTATTTA
AAGAATTAATCTTCTATTTAATGAATTTGAGATTCTTGAATACCTTCTCAATATTCTTGT
TTTATATAAAGGTTCTTCATCATCACATCTCTTGCCTCCTATATTGACACAAGGACAAGA
AATTGGACAACTAAGCGTGGCCTCCCCGATGAATATTTCTCTTCTTTATCGTGAAA
   
Sequence Name 58-K034240-022-764-D11C-KQ05
GenBank Accession LN509630 [GenBank]
Sequencing primer gene specific primer, KQ05: 5'-GCATCTTCTGTTGTTTCACGATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K034240-022-764-D11C-KQ05
ACGCTTAGTTGTCCAATTTCTTGTCCTTGTGTCAATATAGGAGGCAAGAGATGTGATGAT
GAAGAACCTTTATATAAAACAAGAATATTGAGAAGGTATTCAAGAATCTCAAATTCATTA
AATAGAAGATTAATTCTTTAAATAAAAGATTATAAGAGTGTATTTTTATACCGAATTCAT
TAACTTCTCGTAAACGCTTGTTCTTGAGAAAAGATGATCGCGGAACATTTAACGCAGGCA
TTAAATGTTCGATCTCCCAGGGTGACACTTTATTAGGTCTCAAAAATGATGCAAATTCAT
CCCACTGCACCTGCAAAATTAAAGTTAATTAGTGATATGTTATTTATTGCACTCAAAAAC
GTATTCCTAAAAGAATGTTATAAAGTGTGAATGAAAATTTACTTCTAAGCTGCGCCAGTC
TGAACACTTCCAATGAGGAGAGAAATTACTAACTCCAATAATTGTCCCAAAATATCTTAA
TCAATTGAACAAAATGTAAATATGGAGAAAAAGAAAGAGTAATTACCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
AAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAA
GCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCC
Primers used for PCR KQ06 - 8474, show primer details
   
Sequence Name 59-K034240-022-764-D11C-Oril
GenBank Accession LN509919 [GenBank]
Sequencing primer gene specific primer, Oril: unknown
Sequence (A. th genome BLAST matches underlined) 
>59-K034240-022-764-D11C-Oril
AAATTCTGATTTCATCTGATGTTACCTGTCTTTCAGAGAAATCATCACCCTCAAACCGCA
TTGTAAATCTTGAACCGACATTGAACTTATTGTTCACATCGTCTAAGAATTTGACGTAAC
TGACAATAAATTGACTTGACCTGATACTTATAAAAACACAAAATATAAACCTATATGTTG
AACGAACTAATCTATTGATAAATCAATATAATCTAAAATGTGAGTTCAAATGGTTCTTTG
AATTACCTTGGCTTGTAAACCACAATGAACATACATTGGTTATCAAAAGCATGCTTTGCG
GAAGCAATTACCCCATGTCTCATACAATCTATTGATACTATTGATGAAGGTATATTCCCT
TGTTGATGTCTTGCTCGTCTGATACCAACTCGTAACTCTCCAGTCTCTCCCCTAAAACA
   
Sequence Name 60-K034240-022-764-D11C-KQ06
GenBank Accession LN510182 [GenBank]
Sequencing primer gene specific primer, KQ06: 5'-GTTTTAGGGGAGAGACTGGAGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K034240-022-764-D11C-KQ06
CAACAAGGGGAAATACCTTCATCAATAGTATCAATAGATTGTATGAGACATGGGGTAATT
GCTTCCGCAAAGCATGCTTTTGATAACCAATGTATGTTCATTGTGGTTTACAAGCCAAGG
TAATTCAAAGAACCATTTGAACTCACATTTTAGATTATATTGATTTATCAATAGATTAGT
TCCTTCAACATATAGGTTTATATTTTGTGTTTTTATAAGTATCAGGTCAAGTCAATTTAT
TGTCAGTTACGACAAATTCTTAGACGCTGTGAACAATAAGTTCAATGTCGGTTCAAGATT
TACAATGCGGTTTGAGGGTGATGATTTATCTGAAAGAAGGTAACATTAGATGAAAAAGAA
TTTCTGCATAAATATATTTATAATGTCTAATATTATCTATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
CACGTCCCAAATGG
Primers used for PCR 60wl - 8474, show primer details
   
Sequence Name 07-K118545-0022-764-D11C-8409
GenBank Accession LN489480 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K118545-0022-764-D11C-8409
ACAGCGGCTAAGAATTTGTCGTAACTGACAATAAATTGACTTGACCTGATACTTATAAAA
ACACATAATATCCACCTATATGTTGAAGGAACTAATCTATCGATAAATCAATATAATCTA
AAATGTGAGTTCAAATGGTTCTTTGAATTACCTTGGCTTGTAAACCACAATGAACATACA
TTGGTTATCAAAAGCATGCTTTGCGGAAGCAATTACCCCATGTCTCATACAATCTATTGA
TACTATTGATGAAGGTATATTCCCTAGTTGATGTCTTGCTCGTCTGATACCAACTCGTAA
CTCTCCAGTCTCTCCCCTAAAACCACAAGTAAATAGTTTAAAGTCCAAAACTCAAGAACC
AAAAAATATATAACAAGAACCAATATTATACCTAACGAATACAATAACATCTCCTTTAAC
CAATTTTTTTGATGTTGTAAATTCATTCCAACCAGTTGTTAATAAATGTCTTTGCGGTGT
ACCTGCATGGAATAAGAAGATTATTAGTTACTTCTCTAGGATTTATTACTTCAATAGCTG
TGGTTGAATTATCTGAATAAAATGAATTATTACCTCTATAGCTGTGTCTGAAGCTCCATT
GATTACCATGGAGATCTATAGCAAGAAGTTCTTGTGCCGGTAGAGGCTGAGACATATCCA
AAACAAAAATTTAATATGTTATGTCTAATGAAAGCTTAAAATTCAAATTCCTTTAAAAGC
TATAACATTAAATACAAAAGG
   
Sequence Name 43-K118546w-0022-764-D11C-60wl
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 60wl: 5'-GCTAATGGTGTATTTTCTGTTCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K118546w-0022-764-D11C-60wl
ATGTTATAGCTTTTTAACGAATTTGAATTTTAAGCTTTCATTAGACATAACATATTAAAT
TTTTGTTTAGGATATGTCTCAGCCTCTACCGGCACAAGAACTTCTTGCTATAGATCTCCA
TGGTAATCAATGGAGCTTCAGACACAGCTATAGAGGTAATAATTCATTTTATTCAAATAA
TTCAACCACAGCTATTGAAGTAATAAATCCTAGAGAAGTAACTAATAATCTTCTTATTCC
ATGCAGGTACACCGCAAAGACATTTATTAACAACTGGTTGGAATGAATTTACAACATCAA
AAAAATTGGTTAAAGGAGATGTTATTGTATTCGTTAGGTATAATATTGGTTCTTGTTATA
TATTTTTTGGTTCTTGAGTTTTGGACTTTAAACTATTTACTTGTGGTTTTAGGGGAGAGA
CTGGAGAGTTACGAGTTGGTATCAGACGAGCAAGACATCAACAAGGGAATATACCTTCAT
CAATAGTATCAATAGATTGTATGAGACATGGGGTAATTGCTTCCGCAAAGCATGCTTTTG
ATAACCAATGTATGTTCATTGTGGTTTACAAGCCAAGGTAATTCAAAGAACCATTTGAAC
TCACATTTTAGATTATATTGATTTATCAATAGATTAGTTCCTTCAACATATAGGTTTATA
TTTTGTGTTTTTATAAGTATCAGGTCAAGTCAATTTATTGTCAGTTACGACAAATTCTTA
GACGCTGTGAACAATAAGTTCAATGTCGGTTCAAGATTTACAATGCGGTTTGAGGGTGAT
GATTTATCTGAAAGAAGGTAACATTAGATGAAAAAGAATTTCTGCATAAATATATNTATA
ATGTCTAATATTATCTATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTCAATTCAAAATGT

Last Updated on 06.12.2018 16:11