Sequences from confirmation amplicon


Confirmation sequences for line 764G03 and hit At5g22440 are shown.
Line ID 764G03
Locus At5g22440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7435599
Primers used for PCR 75rn - 8474, show primer details
   
Sequence Name 62-K116102-0022-764-G03C-75rn
GenBank Accession LN510833 [GenBank]
Sequencing primer gene specific primer, 75rn: 5'-CTATGACCCTCAAAAGGACAAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K116102-0022-764-G03C-75rn
GGCCAAGATGAAGATTTGCATGCTTGGTGATGCCCAGCACGTCGAAGAGGTTCACTTTTT
TCCGCCTTTTCTTTTGAAAATGTTATGATTAGTACTTAGTAGTATAGTACTATAACTTGC
GGTTGGTTTACTTAGGCTGAGAAGATTGGATTGGAATCTATGGATGTTGAAGCTCTTAAG
AAGTTGAACAAGAACAAGAAACTTGTTAAGAAGCTTGCTAAGAAATTCCATGCTTTCCTT
GCTTCTGAATCTGTCATTAAGCAGATTCCTCGTCTTCTTGGTCCTGGTCTCAACAAGGCA
GGCATGTCTCTAACCTTACCTAATCTTTGCATATGTTCTTGTATCCTCTCTATATGATGC
TACATTAAATCTTGACTCTTATGAACAATGAACAGGAAAATTCCCAACTCTTGTTAGCCA
CCAAGAGTCTTTGGAATCCAAAGTGAATGAGACAAAGGCAACAGTGAAGTATTCAATTGA
CTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCG
   
Sequence Name 44-K116101-0022-764-G03C-8409
GenBank Accession LN505296 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K116101-0022-764-G03C-8409
TTACATTGAATATATCCTGGAGTCAATTGAATACTTCACTGTTGCCTTCTTCTCATTCAC
TTTGGATTCCAAAGACTCTTGGTGGCTAACAAGAGTTGGGAATTTTCCTGTTCATTGTTC
ATAAGAGTCAAGATTTAATGTAGCATCATATAGAGAGGATACAAGAACATATGCAAAGAT
TAGGTAAGGTTAGAGACATGCCTGCCTTGTTGAGACCAGGACCAAGAAGACGAGGAATCT
GCTTAATGACAGATTCAGAAGCAAGGAAAGCATGGAATTTCTTAGCAAGCTTCTTAACAA
GTTTCTTGTTCTTGTTCAACTTCTTAAGAGCTTCAACATCCATAGATTCCAATCCAATCT
TCTCAGCCTAAGTAAACCAACCGCAAGTTATAGTACTATACTACTAAGTACTAATCATAA
CATTTTCAAAAGAAAAGGCGGAAAAAAGTGAACCTCTTCGACGTGCTGGGCATCACCAAG
CATGCAAATCTTCATCTTTGGCCTAGGAACGTGAGGCAATTTCACAGAACCGCTAAATCT
CTTGTCCTT

Last Updated on 06.12.2018 16:11