Sequences from confirmation amplicon


Confirmation sequences for line 764H03 and hit At2g21120 are shown.
Line ID 764H03
Locus At2g21120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9052123
Primers used for PCR 19vy - 8474, show primer details
   
Sequence Name 49-K118278-0022-764-H03C-19vy
GenBank Accession LN506980 [GenBank]
Sequencing primer gene specific primer, 19vy: 5'-AAGCTACCACCGAACAAACTTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K118278-0022-764-H03C-19vy
CGGTGATTTCTACTTGTTATGAATCTAAGAAGTCTTGTCTTCCTTCGTATAACGGACTTA
TCGATCATTCAGATTCTCTAAAATGGTAATATTCATGTTCTCTGTCTCTCTCGTTGATCG
ATTTATAAAGAAACGAAACAGAGATTCTTGTTATTTCTCTCAATTCTGATCAAACTTTTA
CCTTATTATTTCATTCAAGTGAGATACAATGAAGGAATATTTCATCCTAGAAAAATGTAG
ATACATATATAAATATATAACAGTGTAAATTTGGACAAAAGAGTCCAGATAAGAAGAAGA
CCTGTACTGTACTAAGAAGAGAGACACAAAGATCAAAACTAAAGAATGTGAAGTTTTTTT
AACTAGACAAACTTTTTTGTCTGTACAGTGACCATAAAACAATACAAAAATGAACCCGAC
CGCAGATTTGTTGATTGATCCAGTTTTAAGACTCATGAGGCTTGTTTTTGGCAAATTTCA
TTCAATCTATTGAGAGCCTAGAGATATTGCATCTTCTTCTTCCTAGTATTCAGGACTGTA
CAGAACACCCAAAAACAAAGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 17-K118277-0022-764-H03C-8409
GenBank Accession LN494055 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K118277-0022-764-H03C-8409
CATTGAATATCTTTGTTTTTGGGTGTTCTGTACAGTCCTGAATACTAGGAAGAAGAAGAT
GCAATATCTCTAGGCTCTCAATAGATTGAATGAAATTTGCCAAAAACAAGCCTCATGAGT
CTTAAAACTGGATCAATCAACAAATCTGCGGTCGGGTTCATTTTTGTATTGTTTTATGGT
CACTGTACAGACAAAAAAGTTTGTCTAGTTAAAAAAACTTCACATTCTTTAGTTTTGATC
TTTGTGTCTCTCTTCTTAGTACAGTACAGGTCTTCTTCTTATCTGGACTCTTTTGTCCAA
ATTTACACTGTTATATATTTATATATGTATCTACATTTTTCTAGGATGAAATATTCCTTC
ATTGTATCTCACTTGAATGAAATAATAAGGTAAAAGTTTGATCAGAATTGAGAGAAATAA
CAAGAATCTCTGTTTCGTTTCTTTATAAATCGATCAACGAGAGAGACAGAGAACATGAAT
ATTACCATTTTAGAGAATCTGAATGATCGATAAGTCCGTTATACGAAGGAAGACAAGACT
TCTTAGATTCATAACAAGTTAGAAATCTACCGGTGAAATCATTGTAGGATACTAAAGTGA
TAGAAGTTTGTTCG

Last Updated on 06.12.2018 16:11