Sequences from confirmation amplicon


Confirmation sequences for line 765A03 and hit At4g16155 are shown.
Line ID 765A03
Locus At4g16155 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9155054
Primers used for PCR 59gp - 8474, show primer details
   
Sequence Name 51-K112250-0022-765-A03C-59gp
GenBank Accession LN507547 [GenBank]
Sequencing primer gene specific primer, 59gp: 5'-TTTACCAGAAATTCCTTGAGCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K112250-0022-765-A03C-59gp
CATCACCGATGCAGTACAAGTGGGGAACCTATCAACCAAGAGTGACTCAGTAGCTTAAAA
ATATATAACTTTTGGTAAGAAATAAGCTATATGAAAGAAAACTCCACTAACCAGCTTTCC
ATTTCCATCAATAACACGCATTCGCTCATCAACTGGTATAAAACCTCTTTGTGTGGTAAC
ATTGATCTGTGAGGAAATCACAGCTTTTATTATTTCTTTAGTAACCAAATAAGGTCCTCA
GGGAACGAAAATGAAAACATATTCATAAGGAATGGGGTTTATTGTTAATTCATACATTTT
CCAGGCCAAGACCATTGGTGAATGGAGCTCTTCCAGTAGCAATTAGAGCAGCGTCAACCT
TGAACAAGGAACTTATGGTCAAACTTTCAGTGCAGGCAAAAAACACATGAAAGTCTCAAA
TGTGATTGTAACTCCAAGACATTCTATTATTATTATTATTATTTTAATTTTTAGGAAAAG
ATCTGATTTTATAATACGAAAAGCCCTGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
TCCG
   
Sequence Name 41-K112249-0022-765-A03C-8409
GenBank Accession LN504129 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K112249-0022-765-A03C-8409
AAAATCAGATCTTTTCCTAAAAATTAAAATAATAATAATAATAATAGAATGTCTTGGAGT
TACAATCACATTTGAGACTTTCATGTGTTTTTTGCCTGCACTGAAAGTTTGACCATAAGT
TCCTTGTTCAAGGTTGACGCTGCTCTAATTGCTACTGGAAGAGCTCCATTCACCAATGGT
CTTGGCCTGGAAAATGTATGAATTAACAATAAACCCCATTCCTTATGAATATGTTTTCAT
TTTCGTTCCCTGAGGACCTTATTTGGTTACTAAAGAAATAATAAAAGCTGTGATTTCCTC
ACAGATCAATGTTACCACACAAAGAGGTTTTATACCAGTTGATGAGCGAATGCGTGTTAT
TGATGGAAATGGAAAGCTGGTTAGTGGAGTTTTCTTTCATATAGCTTATTTCTTACCAAA
AGTTATATATTTTTAAGCTACTGAGTCACTCTTGGTTGATAGGTTCCCCACTTGTACTGC
ATCGGTGATGCCAATGGTAAACTGATGCTTGCTCATGCAGCTAGTGCTCAAGGA

Last Updated on 06.12.2018 16:11