Genotyping and primer details


Genotyping and primer details for 765A03 - At4g16155
Line ID 765A03
Locus At4g16155 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9155054 of line 765A03 Chr4:9155054
   
Insertion allele amplicon 8474 - 59gp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 59gp: 5'-TTTACCAGAAATTCCTTGAGCACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9154509
   
wt amplicon (over InsPos) 29hs - 59gp
wt amplicon start Chr4:9154509
wt amplicon end Chr4:9155570
wt amplicon size 1062 bp
Locus specific primer 59gp: 5'-TTTACCAGAAATTCCTTGAGCACT-3'
2nd locus specific primer 29hs: 5'-ATTTTGCTTAAGGTTTGTGATGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11