Sequences from confirmation amplicon


Confirmation sequences for line 768F05 and hit At5g65980 are shown.
Line ID 768F05
Locus At5g65980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26392500
Primers used for PCR 25gd - 8474, show primer details
   
Sequence Name 80-K112062-0022-768-F05C-25gd
GenBank Accession LN513965 [GenBank]
Sequencing primer gene specific primer, 25gd: 5'-TAGCTACAGCATGACGATGAGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K112062-0022-768-F05C-25gd
TACCTGAGACATCCCAAAGTCTTTCGTCCCATATTGAATCTTCTAATTTTGATATCTATG
TGATTTTGCATATGCACTACCTGAGAGACCAACAGTACCTAGAGGCCAACTTGTCCTTTT
TATTCACCTTTCTTGCATGACTTTTCCTATGTTGAAAAGTTAGACTCCTCTTTTACACTT
CATGTACCCCACTCCTATACGTATTTAATCTAAATATATATACCCACCAATCCTCTCCAT
CTACACAAACAGCCCAAATATCAAACCTTTCATCTCTATATCTTTAACCTTGACTGCTTC
TCTTCTTCTACAAGAAACCAGAAAAAAAGAAAAGAAAAGAAAAAGAAGAGATTAGGGATG
GGTTTCTTAGAGTTGTTGGAGGTGGCTTCAATGCCAATCGTTCAAGTTCTTCTTGGGGAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATA
   
Sequence Name 64-K112045-0022-768-F05C-8409
GenBank Accession LN511232 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K112045-0022-768-F05C-8409
ATTTACAATTGAATATTCCTGCTGATCCATGTAGATTTCCCGGACATGAAGCCATTTCCC
CAAGAAGAACTTGAACGATTGGCATTGAAGCCACCTCCAACAACTCTAAGAAACCCATCC
CTAATCTCTTCTTTTTCTTTTCTTTTCTTTTTTTCTGGTTTCTTGTAGAAGAAGAGAAGC
AGTCAAGGTTAAAGATATAGAGATGAAAGGTTTGATATTTGGGCTGTTTGTGTAGATGGA
GAGGATTGGTGGGTATATATATTTAGATTAAATACGTATAGGAGTGGGGTANATGAAGTG
TAAAAGAGGAGTGTAANTTTTNAANATAGGAAAAGTNATGNAAGAAAGGTGAATAAAAAG
GANAAGTTGGNNTNTAGGTANTGTTGGTNTNTNAGGTAGTGNATATGNAAAATNANATAG
ATATNAAAATTAGAAGATTNAATATTGGGAAGAAAGAATTTTGGGATTGTNTNAGGTTAA
TAATGTTGANAGAATNAAAGTAA

Last Updated on 06.12.2018 16:11