Sequences from confirmation amplicon


Confirmation sequences for line 769A07 and hit At5g50800 are shown.
Line ID 769A07
Locus At5g50800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20665478
Primers used for PCR NJ76 - 8474, show primer details
   
Sequence Name 73-K036620-022-769-A07C-8409
GenBank Accession LN512845 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K036620-022-769-A07C-8409
CGCCTTCGGTTGTCTTTGGATTATCTGTCTTCTGAGCCACAGGAGTTTTGTAGTATTTGA
AAATTATGTAGAGAATCATTTGAACAGCTCCTAAGAACGCGCCTAATACATTTGGAAGCT
GCAATAGTTCAAAAAACGTGTAAAGGACATTAATATTCCATTTATTGTCGACAAAAGAAG
TTGGCAAAGAAAAATTATAAAAGGGAAATTACCGACATCACAACATAACAAATTAGTTTT
TTTGTTTCTAGTATTTGCTATTGTACTCTCAATTCCTTAGCACATTTAGTGGGGGTCCAA
TTATTTATATAATGCTAATAATAAAGCGAAAAATGTGTACGGTTCAAAATCATATAGTTT
GAATGAGGAAAAAAAAGACAAAGGTAACTACTGTTTTTACTTTATTAAAGAACCACCCAT
AATAATTAATCTAATCATATAATGTTGTGCTGTCCTTTATAATATATTTTGAAATAATCG
AAACAGAATAAAATAAGAGAATTATTTGAGAATTGTATAAATAAACTCACGGCAACGTAG
AAGTCTTTAATAGCGAGACCGTAGAAGAGCCACGTGACGGCGCTAATTGTAAGAAACAAC
GATAAAGAGAAAGGCATAAACTCCACACTTC
   
Sequence Name 74-K036620-022-769-A07C-NJ76
GenBank Accession LN513019 [GenBank]
Sequencing primer gene specific primer, NJ76: 5'-AGAAGTGTGGAGTTTATGCCTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K036620-022-769-A07C-NJ76
CGCCGTCCGTGGCTCTTCTACGGTCTCGCTATTAAGACTTCTACGTTGCCGTGAGTTTAT
TTATACAATTCTCAAATAATTCTCTTATTTTATTCTGTTTCGATTATTTCAAAATATATT
ATAAAGGACAGCACAACATTATATGATTAGATTAATTATTATGGGTGGTTCTTTAATAAA
GTAAAAACAGTAGTTACCTTTGTCTTTTTTTTCCTCATTCAAACTATATGATTTTGAACC
GTACACATTTTTCGCTTTATTATTAGCATTATATAAATAATTGGACCCCCACTAAATGTG
CTAAGGAATTGAGAGTACAATAGCAAATACTAGAAACAAAAAAACTAATTTGTTATGTTG
TGATGTCGGTAATTTCCCTTTTATAATTTTTCTTTGCCAACTTCTTTTGTCGACAATAAA
TGGAATATTAATGTCCTTTACACGTTTTTTGAACTATTGCAGCTTCCAAATGTATTAGGC
GCGTTCTTAGGAGCTGTTCAAATGATTCTCTACATAATTTTCAAATACTACAAAACTCCT
GTGGCTCAGAAGACAGATAAATCCAAAGACAACCGAAGGCGGGAAACCGAAGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11