Sequences from confirmation amplicon


Confirmation sequences for line 769C10 and hit At5g54840 are shown.
Line ID 769C10
Locus At5g54840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22277193
Primers used for PCR 05iy - 8474, show primer details
   
Sequence Name 88-K113077-0022-769-C10C-05iy
GenBank Accession LN514881 [GenBank]
Sequencing primer gene specific primer, 05iy: 5'-AATCGTAGTCGATAATGGGTTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K113077-0022-769-C10C-05iy
TCAGGCAATTAAAAGTCTAGCCAAATGAATTTGAAGATCTTGTTCACATTTATGTTGTGT
GTCGCACTTGAGAAGAACAATGACGCATTCATCACCTTCGCATATGCTCTTGCCTAATAA
ATCATATAGAACCAAATTAACTATTTTTTTATAGAAATGAATTATCACAATAAATCGAAA
TGTCTAAATCATCATGAAAACGTAAAATATGAAAAGGGTCTTAATTGGTAACGTGGTATG
ATTAAGTTGTATGATTGGTATTACCTGAGTGACGATGGTCCACTGAAGATTGGGTGGGAG
CCGAACAAAGTCATCGAATTTTGTTCCTATTAGAATCGGAATTGCCGTCTGATTAAAGAA
AACACAAAATTATAAAAAAAAGTTTTAAGATAAAGTATTTTGTTGGAATCAGATTCCAAA
AACTTTATAATATACTCCTTGAATGTTAACAACTAATAAGAAAAGTCGACGTGCTAAATT
TTATTTAGTTCAGGCGAAACTAAAACGAAAAACAGGAACCAATTTTAAATCTACATGAAT
CAATATGGCTTCAATATGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 61-K113075-0022-769-C10C-8409
GenBank Accession LN510568 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K113075-0022-769-C10C-8409
ATTGATTCTGTAGATTTAAATTGGTTCCTGTTTTTCGTTTTAGTTTCGCCTGAACTAAAT
AAAATTTAGCACGTCGACTTTTCTTATTAGTTGTTAACATTCAAGGAGTATATTATAAAG
TTTTTGGAATCTGATTCCAACAAAATACTTTATCTTAAAACTTTTTTTTATAATTTTGTG
TTTTCTTTAATCAGACGGCAATTCCGATTCTAATAGGAACAAAATTCGATGACTTTGTTC
GGCTCCCACCCAATCTTCAGTGGACCATCGTCACTCAGGTAATACCAATCATACAACTTA
ATCATACCACGTTACCAATTAAGACCCTTTTCATATTTTACGTTTTCATGATGATTTAGA
CATTTCGATTTATTGTGATAATTCATTTCTATAAAAAAATAGTTAATTTGGTTCTATATG
ATTTATTAGGCAAGAGCATATGCGAAGGTGATGAATGCGTCATTGTTCTTCTCAAGTGCG
ACACACAACATAAATGTGAACAAGATCTTCAAATTCATTTTGGCTAGACTTTTTAATTTG
CCTTGGAAGATTGACAGAAATTTGACCTTAGGTGAACCCATTAT

Last Updated on 06.12.2018 16:11