Sequences from confirmation amplicon


Confirmation sequences for line 769G05 and hit At4g39460 are shown.
Line ID 769G05
Locus At4g39460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18356871
Primers used for PCR 61by - 8474, show primer details
   
Sequence Name 84-K108479-0022-769-G05C-61by
GenBank Accession LN514442 [GenBank]
Sequencing primer gene specific primer, 61by: 5'-CGCTCCTTAACTAATGCCAGATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K108479-0022-769-G05C-61by
CTCGGTTTAGATGAATGGATCCATCGTACTGTGTACTTTTAAGGTAAATGCAGACCAGAG
CCTTTTTTTTTAAGTTCAGCGGCTTAATTCTCATTCAGGTTTGATTAAATGGATCCGAAG
AATATATATTAAAGCATATAAGGTATGTCACCAACCAGCAAATGCACCGATTAGAGCGTT
CTCAGGATCACTAAGCTCTCTACGCGCCTGCCAAAGAGTTGACCAAAGTAAATCTCATGC
AAAATATCAGACATGAATGTATACACGTTATATTTAGGGTCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATA
   
Sequence Name 72-K108478-0022-769-G05C-8409
GenBank Accession LN512738 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K108478-0022-769-G05C-8409
TTGAATTATCCTGACCCTAAATATAACGTGTATACATTCATCTTTGATATTTTGCATGAG
ATTTACTTTGGTCAACTCTTTGGCAGGCGCGTAGAGAGCTTAGTGATCCTGAGAACGCTC
TAATCGGTGCATTTGCTGGTTGGTGACATACCTTATATGCTTTAATATATATTCTTCGGA
TCCATTTAATCAAACCTGAATGAGAATTAAGCCGCTGAACTTAAAAAAAAAAGGCTCTGG
TCTGCATTTACCTTAAAAGTACACAGTACGATGGATCCATTTCATCTAAAACCGAGTTAA
TCATAGTGTGCAAGTGATGTATCTGGCATTAGTTAAGGAGCG

Last Updated on 06.12.2018 16:11