Genotyping and primer details


Genotyping and primer details for 769G05 - At4g39460
Line ID 769G05
Locus At4g39460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:18356871 of line 769G05 Chr4:18356871
   
Insertion allele amplicon 8474 - 61by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 61by: 5'-CGCTCCTTAACTAATGCCAGATAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:18356574
   
wt amplicon (over InsPos) 05dt - 61by
wt amplicon start Chr4:18356574
wt amplicon end Chr4:18357454
wt amplicon size 881 bp
Locus specific primer 61by: 5'-CGCTCCTTAACTAATGCCAGATAC-3'
2nd locus specific primer 05dt: 5'-TACTCTCGCATATGCCTTGTATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11