Sequences from confirmation amplicon


Confirmation sequences for line 770D07 and hit At1g77890 are shown.
Line ID 770D07
Locus At1g77890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29295352
Primers used for PCR 41op - 8474, show primer details
   
Sequence Name 42-K114909-0022-770-D07C-41op
GenBank Accession LN504567 [GenBank]
Sequencing primer gene specific primer, 41op: 5'-GTCACCATATTTACTCCATTGCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K114909-0022-770-D07C-41op
TTTTTTAAGGGTATCACTAGAATTGAGGAAGCCTCGGGTATGAACAAATGACATATCGAC
AATCTGGGTTCCTGCCAAAATGTATTGTCCTGTTTTCGTGAATCACGGGTATTAAAATAA
ACGTGTCGATAGGAATTAAAGAAAAGTAGAACTTGTTTACCTTGAAAGGATCAATACTTG
TTGGGATGATTAGGTTGACGTGAAGAATGAGGATACTCAACCAAATTCCATTCACCGATA
GAGTTTTTATCCTTTTTAACATCAGGAAACTCCACCATGTAGCTTGGATCTCGATTCGGA
ACATTATAATAGCTACTGTTCCACTGCAAGCCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCCA
   
Sequence Name 18-K114908-0022-770-D07C-8409
GenBank Accession LN494422 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K114908-0022-770-D07C-8409
TTAAAAAGGATAAAAACTCTATCGGTGAATCGGAATTTGGTTTGAGTATCCTCATTCTTC
ACGTCAACCTAATCATCCCAACAAGTATTGATCCTTTCAAGGTAAACAAGTTCTACTTTT
CTTTAATTCCTATCGACACGTTTATTTTAATACCCGTGATTCACGAAAACAGGACAATAC
ATTTTGGCAGGAACCCAGATTGTCGATATGTCATTTGTTCATACCCAAGGCATCCTCAAT
TCTAGTGATACCTTAGAAAAACCACATATGGAGCTTTATTATTTAAATCTTTGATACCTT
CTTCTGTAATTATGAAACACAGATTATTATGTAAATTAGCAGGCAATGGA

Last Updated on 06.12.2018 16:11