Sequences from confirmation amplicon


Confirmation sequences for line 771C01 and hit At4g05520 are shown.
Line ID 771C01
Locus At4g05520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2807240
Primers used for PCR 25vd - 8474, show primer details
   
Sequence Name 25-K117753-0022-771-C01C-25vd
GenBank Accession LN497640 [GenBank]
Sequencing primer gene specific primer, 25vd: 5'-CCGCTTTTTCTAAAGCTAATGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K117753-0022-771-C01C-25vd
CAGAGGTCGTACGTGTCTATATTGGGTATGTTTTTTCGAGTACAACATGAATTGTCAACA
AAGAAGTCAAAAAGCCATTAGTTTCACTTGTACACACGATAGATTGATTTCTTTTTCTTT
GCTTTTAACTTGTGCAGGTCCTTCAATGACAAACCCATAAACGAAGTTGCAGTTGGACCG
ATTGGGAAAGAACTCTTTGAGAAAGAGCAAAATGATCTTCTAGCAGACTTAATGGACGTA
CCCAAGAAAGCATGTGACAGAAAGGTATGATTTTGATGAGTCTCAATTTAAACAATAATG
TATGTTACAATTCTCAAATCCTGACTATTTTTTTTGTTATTCTTTGCAGATTAATGAGTT
TGTGAAGCGAGCTCGATCTGGAAGATGAATCTTCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATC
   
Sequence Name 09-K117752-0022-771-C01C-8409
GenBank Accession LN490378 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K117752-0022-771-C01C-8409
CTGAAGAATTCATCTTCCAGATCGAGCTCGCTTCACAAACTCATTAATCTGCAAAGAATA
ACAAAAAAAATAGTCAGGATTTGAGAATTGTAACATACATTATTGTTTAAATTGAGACTC
ATCAAAATCATACCTTTCTGTCACATGCTTTCTTGGGTACGTCCATTAAGTCTGCTAGAA
GATCATTTTGCTCTTTCTCAAAGAGTTCTTTCCCAATCGGTCCAACTGCAACTTCGTTTA
TGGGTTTGTCATTGAAGGACCTGCACAAGTTAAAAGCAAAGAAAAAGAAATCAATCTATC
GTGTGTACAAGTGAAACTAATGGCTTTTTGACTTCTTTGTTGACAATTCATGTTGTACTC
GAAAAAACATACCCAATATAGACACGTACGACCTCTGGTGTGTTCAGGACTTTACCAAGC
GACCACATCAATGCTCCATACACTCTCATTAGCTTAGAAAAAA

Last Updated on 06.12.2018 16:11