Sequences from confirmation amplicon


Confirmation sequences for line 773C06 and hit At1g54510 are shown.
Line ID 773C06
Locus At1g54510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20361335
Primers used for PCR HN04 - 8474, show primer details
   
Sequence Name 33-K035981-022-773-C06C-8409
GenBank Accession LN500908 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K035981-022-773-C06C-8409
GCCATTTAAATTGAAATATCCTGATGCACACCTCTTCCTGAAAATGCACACCATTGGATT
TCTTGATAGCTTGTGCCCTGTAACATAAGAAGAATATGAGGCTGCGTCACCATCTATATA
GTGAAGCCAAAATGACAGAATCTACGTAAAAAAAAAAGCTGAACCACAGAAAACTAACAT
GTCTCCTCCTTCACAATAACCTATGACAATGCACACATAGCAGGCCTAAAAGAAAGAATA
CAAAATCCTTATTAGCTCAAAAGGTGTGGAATGATATATACTTTTGTTCAAGTCATAAGG
TATGCCTTACCTTTTCCACCCAAGAGTCTTTGTATTCCACGATAAATGGATGACGCATTT
TCGAGATAAGCTCCATCTACTCAAAGAAATGAAACTAAGATCAAACCATAGAAAAAAGCT
TATCATACAGAGAGTATATAGAAAAACACCAACCTCTTGATGTGCAGATCGTCTGGTCCT
TTGAGTTTGCCGGGCAAGACGGATCTTTTTCAATACATATCTAATGAAAACCAATACATG
ACATCAAAAATTTAAAACTTTCTTGAAGTAAAACAATAAGAGGCAGCAAG
   
Sequence Name 34-K035981-022-773-C06C-HN04
GenBank Accession LN501324 [GenBank]
Sequencing primer gene specific primer, HN04: 5'-GCTATCTTCTTTTGCTGCCTCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K035981-022-773-C06C-HN4
ATGTATTGGTTTTTCATTAGATATGTATTGAAAAAGATCCGTCTTGCCCGGCAAACTCAA
AGGACCAGACGATCTGCACATCAAGAGGTTGGTGTTCTTCTATATACTCTCTGTATGATA
AGCTTTTTTCTATGGTTTGATCTTAGTTTCATTTCTTTGAGTAGATGGAGCTTATCTCGA
AAATGCGTCATCCATTTATCGTGGAATACAAAGACTCTTGGGTGGAAAAGGTAAGGCATA
CCTTATGACTTGAACAAAAGTATATATCATTCCACACCTTTTGAGCTAATAAGGATTTTG
TATTCTTTCTCTTAGGCTTGCTATGTGTGCATTGTCATAGGTTATTGTGAAGGAGGAGAC
ATGTTAGTTTTCTGTGGTTCAGCTTTTTTTTTTACGTAAATTCTGTCATTTTGGCTTCAC
TATATAGATGGTGACGCAGCCTCATATTCTTCTTATGTTACAGGGCACAAGCTATCAAGA
AATCCAATGGTGTGCATTTTCAGGAAGAGGTGTGCATCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACTTCCTCCC

Last Updated on 06.12.2018 16:11